The Versatility of Recombinant Bacterial Proteins and cDNA

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Recombinant Bacterial Proteins and cDNA

Recombinant bacterial proteins and cDNA are important tools in molecular biology and biotechnology that are used for a variety of purposes. Recombinant proteins are artificially produced proteins that are created by combining DNA from different sources, while cDNA is a synthesized copy of the messenger RNA (mRNA) molecule that carries the genetic information from a gene. These tools have numerous applications in research and medicine, particularly in the development of vaccines, diagnostic tests, and as research tools.

Recombinant bacterial proteins can be used to create modified bacterial proteins that can be studied to better understand bacterial infections and develop effective treatments. They can also be used as research tools to study protein interactions, protein structure and function, and for drug development. cDNA, on the other hand, is used to produce a corresponding DNA sequence that can be inserted into a host organism, such as bacteria or yeast, to produce a desired protein. This is particularly useful when the original gene is difficult to isolate or express in its natural form.

One important application of recombinant bacterial proteins and cDNA is in the development of vaccines. By producing recombinant bacterial proteins, researchers can create vaccines that stimulate the immune system to produce an immune response against the bacteria. This can be particularly useful for bacteria that are difficult to grow in the laboratory or that do not produce a strong immune response.

Recombinant bacterial proteins and cDNA are also used in the development of diagnostic tests for bacterial infections. By producing recombinant proteins or using cDNA to identify the presence of bacterial DNA, researchers can develop sensitive and specific tests that can diagnose bacterial infections quickly and accurately.

The use of recombinant proteins and cDNA has grown significantly in both academic research and therapeutic applications. However, successful expression of recombinant proteins in heterologous systems depends on various factors, such as codon preference, RNA secondary structure, and GC content. Optimizing these factors can increase expression levels by up to a hundred times compared to pre-optimization. Bioclone has developed a proprietary technology platform resulting in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones cloned in E.coli expression Vector, which are readily available for the production of recombinant proteins.

Bioclone offers a diverse selection of recombinant antigens and codon-optimized cDNA derived from a multitude of bacterial sources.

cDNA and recombinant antigen

Codon-optimized cDNA is cloned into E. coli expression vector with 6x His-tag at N-terminus and ready-to-use for recombinant protein production.
Recombinant protein applications: Western Blot may be used for other applications determined by the user.
Protein Purity: >90%, as determined by SDS-PAGE under reducing conditions.
Protein Activity: N/A
Protein Tag: Contains A 6x histidine tag.
Protein Formulation: Liquid
Source: Produced from E. coli