Streptococcus gordonii cDNA and recombinant antigen

Streptococcus gordonii is a type of bacteria that produces various antigens on its outer surface, including surface protein A, surface protein B, and Surface antigen SCP-like domain. These antigens are essential for the bacteria to interact with the host’s body and cause infections.

Surface protein A helps Streptococcus gordonii attach to the host’s cells, allowing it to establish an infection. Surface protein B, on the other hand, helps the bacteria to evade the host’s immune system by preventing the production of antibodies against it. Surface antigen SCP-like domain is involved in the formation of biofilms, which enable the bacteria to stick to surfaces and form a protective layer against the host’s immune system and antibiotics.

Understanding the function of these antigens is crucial in the development of effective treatments and vaccines against Streptococcus gordonii infections. By targeting these antigens, it may be possible to prevent the bacteria from causing infections and reduce the risk of antibiotic resistance.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The use of cDNA and recombinant antigens derived from Streptococcus gordonii can have various applications in the fields of molecular biology, biotechnology, and dentistry. Some of these applications include:

Diagnostic tests: Recombinant antigens can be used in the development of rapid diagnostic tests for the detection of Streptococcus gordonii in clinical samples. These tests can help to quickly identify the presence of the bacteria in the oral cavity and provide early treatment for infected individuals.

Study of bacterial virulence: The use of cDNA from Streptococcus gordonii can help researchers to study the genetic basis of the bacteria’s virulence. By analyzing the cDNA, researchers can identify the genes and gene products involved in the bacteria’s ability to cause disease, which can lead to the development of new treatments and preventions.

Study of bacterial evolution: The use of cDNA from Streptococcus gordonii can help researchers to study the evolution of the bacteria. By analyzing the cDNA, researchers can identify genetic changes that occur in the bacteria over time, which can provide insights into the evolution of oral bacteria and the development of new treatments.

Study of the oral microbiome: Streptococcus gordonii is a common member of the oral microbiome, which is the collection of microorganisms that inhabit the human oral cavity.

The use of cDNA and recombinant antigens can help researchers to study the role of Streptococcus gordonii in the oral microbiome and its interactions with other microorganisms.