Products

One-Step DNA Purification

The BcMag™ One-step DNA Purification System uses negative chromatography magnetic beads to quickly extract and purify DNA from a wide range of biological samples, including blood, animal cells, plants, body fluids, stains, swabs of body fluids, stripped cells, cigarette butts, hair, fingernail scrapings, epithelial cells, bite marks, semen, and touch DNA samples. The magnetic beads in this system have a proprietary surface chemistry that allows them to simultaneously lyse cells and capture PCR inhibitors when mixed with the sample. The magnetic beads-PCR inhibitor complex can then be easily removed using a magnet, leaving behind pure DNA that is ready for downstream analysis.

How It Works

  1. Add functional magnetic beads to the sample.
  2. Mix the samples with the magnetic beads and proteinase K to lyse the cells.
  3. Mix by vortexing/pipetting for the beads to capture the PCR inhibitors.
  4. Remove the beads with a magnet.
  5. Aspirate the supernatant containing the pure ready-to-use DNA/RNA.
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Performance

The purified DNA is suitable for use in sensitive downstream applications, such as PCR, qPCR, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, genotyping, or next-generation sequencing (NGS), buccal DNA sample collection, veterinary genotyping and diagnostics, research genotyping, Veterinary genotyping and diagnostics, genetic testing, forensics, population studies.

Features and Advantages

Consistent accuracy, reproducibility, and sensitivity with a trace amount of sample.

DNA extraction-free

One-Step, One-Tube, No liquid transfer.

No DNA damage, loss, and higher yields of higher purity.

Process hundreds of samples in less than 30 minutes.

Effectively removes inhibitors: polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca2+, Mg2+, etc. (See picture “PCR inhibitor removal”).

Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and organic reagents.

High-throughput: Compatible with many different automated liquid handling systems.

PCR inhibitor removal by magnetic beads

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