One-Step NGS Cleanup Kit

BcMag™ One-Step NGS Cleanup Kit is specially designed for ultrafast and efficient purification of DNA after adaptor ligation and PCR or possible replacement of size selection procedure after adaptor addition. The protocol is not only straightforward (one tube and one step, as shown in Fig.1) but also very flexible in removing different size DNA fragments by adjusting processing time, buffer’s pH, and detergent concentration (table1). The magnetic Beads are added directly to the finished PCR reactions or other DNA reactions and mixed by a vortex mixer or pipetting to capture and remove the impurities (e.g., excess primer, dimer, adapter, salt, detergent, dNTPs, and enzyme). After mixing, the beads are magnetically removed, while the supernatant contains the purified and ready-to-run DNA. In just 1 minute, the purified DNA is ready for downstream applications, such as Sanger Sequencing, Restriction Digestion, Cloning, SNP Detection, or Library Preparation for NGS. The beads enable 96 samples to be processed simultaneously in less than 10 minutes.