- +1 858 909 0079
- +1 858 909 0057
- support@bioclone.net
- +1 858 909 0079
- support@bioclone.net
Gene Synthesis Service
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Fast Turnaround Time: 10-15 business days
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Optimize Codon Usage: Free
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Quality: 100% match with your requested sequences
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Flexibility: Free to clone the synthesized gene into any of your desired vectors
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Products: Final products (2 μg plasmid or glycerol stock with detailed datasheet) will be delivered.
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Lower Price: Call or email for a quotation
Recombinant Protein Production and Purification
E.coli Expression System
Phase I: Expression vector preparation
1. If the customer provides cDNA
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Subcloning your interest cDNA into a bacterial expression vector with His-tag or GST-tag or customer’s expression vector.
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Mini-analysis of the expression of the gene in two different bacterial expression strains and three different temperatures (16˚ C, 28˚, and 37˚C) by SDS-PAGE or Western blot to check protein solubility, stability, and expression level.
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Estimated time: 5-10 Business days
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Price: $1,200
2. If the customer does not provide cDNA
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Bioclone will artificially synthesize your interest cDNA, which will be codon-optimized for maximum expression (Normally, expression level > 2-100 times higher than the original cDNA). Clone into an expression vector.
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Mini-analysis of the expression of the gene in two different bacterial expression strains and three different temperatures (16˚ C, 28˚ C and 37˚C) by SDS-PAGE or Western blot to check protein solubility, stability and expression level.
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Estimated time: 5-10 Business days
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Price: $1.45 /bp (if cDNA <500bp, $600 will be charged for the set-up fee)
Phase II: Large-scale culture and purification
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Affinity purification from: 0.5 liters fermented bacterial culture/$590 (Purity>90%)
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Affinity purification from: 1.0 liter fermented bacterial culture/$890 (Purity>90%)
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Estimated time: 5 business days
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The customer will get two µg plasmids, recombinant protein, and a detailed datasheet.
Conjugation to Magnetic Beads
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Just send us your protein, peptide, or DNA or Oligo-nucleotides or Antibody, and we will conjugate them to our magnetic Beads.
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Price: 30 mg of magnetic beads/$1200
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Turnaround time: 1 week
Bulk and OEM Manufacturing
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OEM magnetic beads for the isolation of nucleic acid from the biological sample
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OEM magnetic beads for biological sample preparation