Propionibacterium acnes cDNA and Antigen

Propionibacterium acnes (P. acnes) is a type of bacteria that commonly resides on the skin, particularly in the sebaceous gland. It is a well-known factor in the development of acne, but research also suggests that it plays a role in the maintenance of skin health.

18 kDa Antigen 2: A Key Player in Immune Response

P. acnes 18 kDa antigen 2 is a protein that has been identified as a key player in the immune response. It activates the production of pro-inflammatory cytokines, which play a crucial role in the body’s immune response to infections. It also helps to activate T-cells, which are white blood cells that play a crucial role in the body’s immune system.

Membrane-Spanning Protein: Maintaining Skin Integrity

The membrane-spanning protein of P. acnes plays a crucial role in maintaining the integrity of the skin. It has been found to help prevent the loss of water from the skin, which can lead to dryness and damage. It also helps to maintain the skin’s pH levels, which is essential for preventing the growth of harmful bacteria.

Myosin-Crossreactive Antigen: A Potential Treatment for Multiple Sclerosis

Research suggests that myosin-crossreactive antigen (MCRA) may have potential as a treatment for multiple sclerosis (MS). MCRA has been found to have an immune-regulatory effect, meaning it can help to suppress the autoimmune response that leads to the development of MS. This protein could be a promising avenue for future MS treatments.

Immunogenic Protein Antigen 84: Potential Use in Vaccines

Immunogenic protein antigen 84 (IPA-84) is a protein that has been found to stimulate the production of antibodies in the body. This makes it a potential candidate for use in vaccines. Research is currently underway to determine whether IPA-84 could be used in a vaccine to protect against P. acnes infections.

RfbX: Exporting O-Antigen for Bacterial Survival

RfbX is a protein that is involved in the export of O-antigen, a molecule that helps bacteria to evade the immune system. This protein is essential for the survival of P. acnes, as it allows the bacteria to maintain its protective barrier and evade detection by the immune system.

J139 60 kDa Chaperonin: Facilitating Protein Folding

J139 60 kDa chaperonin is a protein that helps to facilitate the folding of other proteins. It works by creating a protective environment where proteins can fold without being disrupted by other molecules. This protein is essential for the proper functioning of P. acnes and plays a crucial role in maintaining the overall health of the bacteria.

In conclusion, the proteins of P. acnes play a crucial role in human health. From immune response activation to maintaining skin integrity, these proteins are vital for the proper functioning of the bacteria and the overall health of the body. With ongoing research, it is likely that we will continue to discover new ways in which these proteins can be harnessed to improve human health.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The cDNA and recombinant antigen can be used in various applications for diagnosis, research, and vaccine development.

Diagnostic Tests: cDNA of P. acnes can be used in molecular diagnostic tests to detect the presence of the bacterium in a patient’s sample. This can be done by amplifying a specific genetic target using polymerase chain reaction (PCR) and detecting the amplified product using fluorescence or other methods.

Research: cDNA of P. acnes can be used in research studies to investigate the genetic characteristics and pathogenesis of the bacterium. Recombinant antigens can also be used to study the immune response to P. acnes infections, to identify potential vaccine candidates, and to develop new diagnostic tests.

Vaccine Development: Recombinant antigens of P. acnes can be used to develop vaccines against the bacterium. These vaccines can stimulate the production of specific antibodies that recognize and neutralize P. acnes.