Mycobacterium marinum cDNA and Antigen

Mycobacterium marinum is a bacterium that causes granulomatous skin infections in humans and aquatic animals. It is closely related to Mycobacterium tuberculosis, the bacterium that causes tuberculosis in humans. M. marinum has a unique ability to grow at lower temperatures, making it well adapted to aquatic environments and capable of infecting both fish and humans.

Culture filtrate antigens (CFAs) are proteins that are secreted by M. marinum into the surrounding culture medium during growth. These CFAs play a crucial role in the pathogenesis of M. marinum by interacting with the host immune system and facilitating the bacterium’s survival and replication within host tissues. Several key CFAs of M. marinum have been identified and studied for their roles in the infection process.

10 kDa Culture Filtrate Antigen: The 10 kDa culture filtrate antigen of M. marinum is a secreted protein that has been implicated in the modulation of the host immune response. It has been shown to stimulate the production of pro-inflammatory cytokines and chemokines in infected host cells, leading to inflammation and recruitment of immune cells to the site of infection.

29 kDa Antigen Cfp29: Cfp29 is a protein that is secreted by M. marinum and has been shown to be required for the bacterium’s ability to replicate and survive within macrophages, which are immune cells that are key players in the host defense against bacterial infections. Cfp29 is thought to play a role in the manipulation of host immune responses and the establishment of M. marinum infection.

6 kDa Culture Filtrate Antigen: The 6 kDa culture filtrate antigen of M. marinum is a secreted protein that has been shown to be involved in the regulation of host immune responses. It has been suggested that this antigen may play a role in the evasion of host immune defenses and the establishment of persistent infection.

Antigen 85-A, Cfp2, Cfp6, Pra, Secreted Antigen 85-B FbpB, Secreted Antigen 85-C FbpC_2: These antigens are part of the Antigen 85 complex, which is a family of mycolyl transferases that are involved in the biosynthesis of the mycobacterial cell wall. These antigens are critical for the integrity and stability of the mycobacterial cell wall, and they have been shown to be important for the survival and virulence of M. marinum.

40 kDa Antigen: The 40 kDa antigen of M. marinum is a secreted protein that has been implicated in the host-pathogen interaction. It has been suggested to play a role in the modulation of the host immune response and the establishment of M. marinum infection.

Mpb51 Antigen Protein FbpD: Mpb51 is a protein that is secreted by M. marinum and has been shown to be involved in the modulation of the host immune response. It has been suggested that Mpb51 may play a role in the evasion of host immune defenses and the establishment of persistent infection.

Soluble Secreted Antigen Mpt53: Mpt53 is a secreted protein of M. marinum that has been shown to be involved in the survival and replication of the bacterium within macrophages. It has been suggested to play a role in the manipulation of host immune responses and the establishment of M. marinum infection.

Understanding the functions and roles of these CFAs of M. marinum can provide valuable insights into the mechanisms underlying M. marinum infection and potentially identify new targets for diagnostics and therapeutics. For example,

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The application of cDNA (complementary DNA) and recombinant antigens derived from M. marinum has been of interest for the development of diagnostic and vaccine tools for the control of infections caused by this bacterium.

Diagnostics: cDNA from M. marinum can be used to develop molecular diagnostic tools such as PCR (Polymerase Chain Reaction) assays for the rapid and specific detection of the bacterium in infected individuals. This is especially useful in cases where traditional culture methods are not feasible or are slow.

Vaccine development: Recombinant antigens of M. marinum have been investigated as potential vaccine candidates, but the development of a vaccine for infections caused by this bacterium is still in its early stages. More research is needed to determine the efficacy and safety of these antigens as vaccines.

Overall, the application of cDNA and recombinant antigens of M. marinum has the potential to contribute to the control and prevention of infections caused by this bacterium, which will benefit human and animal health by reducing the incidence of these infections.