Enterobacter sakazakii cDNA and Antigen

Enterobacter sakazakii is a Gram-negative bacterium that is known to cause infections, particularly in neonates and immunocompromised individuals. It produces several proteins that are of interest for research and diagnostic purposes. Here are brief descriptions of the proteins you mentioned:

OmpA: This is an outer membrane protein that plays a role in cell adhesion and invasion. It is a target for vaccine development and diagnostic tests.
Assembly factor yaeT: This is a protein that is involved in the assembly of outer membrane proteins in Gram-negative bacteria. It is important for the stability and integrity of the bacterial cell wall.

UDP-N-acetyl-D-mannosaminuronic acid transferase: This is an enzyme that is involved in the biosynthesis of lipopolysaccharides, which are important components of the outer membrane in Gram-negative bacteria. It is a potential target for antibiotic development.

Cpn60 groEL protein: This is a chaperonin protein that is involved in protein folding and assembly. It is a highly conserved protein that is found in a wide range of bacteria and is often used as a target for diagnostic tests.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

One application of E. sakazakii cDNA is in the development of rapid and accurate diagnostic tests. By sequencing and analyzing cDNA libraries, researchers can identify genes that are specific to this bacterium, which can be used as targets for the development of diagnostic assays.

Recombinant antigens derived from E. sakazakii have been used in the development of immunoassays for the detection of this bacterium in food and environmental samples. These assays can help to ensure the safety of food products and prevent the spread of infections caused by E. sakazakii.

Another application of E. sakazakii cDNA and recombinant antigens is in the study of the pathogenesis of infections caused by this bacterium. By analyzing the genetic makeup of E. sakazakii, researchers can gain insights into the mechanisms of virulence and identify potential targets for the development of therapeutic interventions.