Streptococcus sanguinis cDNA and recombinant antigen

Streptococcus sanguinis is a type of bacteria that produces various surface antigens, some of which include the cell surface SD repeat antigen, staphylococcal antigen A, and GbpB-SagA. These antigens are crucial in the bacterium’s ability to attach to and colonize host tissues, evade the host immune system, and form biofilms.

The SD repeat antigen plays a significant role in the bacterium’s adherence to host tissues. Staphylococcal antigen A is an immunodominant virulence factor that helps S. sanguinis to evade the host immune system. On the other hand, GbpB-SagA is involved in bacterial colonization and biofilm formation.

All these antigens contribute to the pathogenicity of S. sanguinis and are potential targets for vaccine development. By understanding the function of these antigens, researchers can develop effective vaccines and treatments against S. sanguinis infections.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The cDNA and recombinant antigen of Streptococcus sanguinis can be used to identify and quantify the presence of this bacterium in the oral cavity. This can be done using PCR-based methods or ELISA. Additionally, these tools can be used to monitor the effectiveness of treatments targeted at the bacterium, such as antibiotics or probiotics. Furthermore, they can be used to evaluate the impact of environmental factors on the presence of Streptococcus sanguinis in the oral cavity, such as the use of tobacco or alcohol. Finally, these tools can be used to assess the risk of a person for developing diseases associated with the presence of Streptococcus sanguinis in the oral cavity, such as dental caries or periodontal disease.