Photobacterium angustum cDNA and Antigen

Photobacterium angustum is a gram-negative bacterium that is known to cause infections in marine animals. The bacterium possesses various proteins on its surface that are involved in the adhesion process, allowing it to colonize host tissues and cause infections. In this article, we will discuss two of the major surface proteins of Photobacterium angustum – surface antigen and OmpA.

Surface Antigen
The surface antigen of Photobacterium angustum is a major surface protein that is involved in the adhesion process of the bacterium. This protein has been shown to induce immune responses in marine animals, and it is considered a potential vaccine candidate. The antigenicity of this protein makes it an attractive target for further research into potential vaccine development against Photobacterium angustum infections.

OmpA
The OmpA (outer membrane protein A) of Photobacterium angustum is a surface protein that plays a crucial role in the adhesion process of the bacterium. This protein has been shown to be involved in the formation of biofilms, which are complex communities of bacteria that adhere to surfaces. Biofilms can protect bacteria from the host immune system and antibiotics, making them difficult to treat. Understanding the role of OmpA in the biofilm formation of Photobacterium angustum could lead to new strategies for controlling bacterial infections.

In conclusion, surface antigen and OmpA are two major surface proteins of Photobacterium angustum that play a critical role in the adhesion process of the bacterium. These proteins have significant potential in vaccine development and the control of bacterial infections. Further studies are needed to explore the full potential of these proteins and their mechanisms of action in the pathogenesis of Photobacterium angustum infections.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The application of Photobacterium angustum cDNA and recombinant antigen in the detection of Photobacterium angustum is an important tool for the diagnosis and identification of this bacterium. cDNA and recombinant antigens can be used to detect the presence of Photobacterium angustum in clinical samples, such as blood and tissue samples. cDNA can be used to create a PCR assay to detect the presence of the bacteria in a sample, while recombinant antigens can be used in an enzyme-linked immunosorbent assay (ELISA) to detect the presence of Photobacterium angustum in a sample. Both methods are highly sensitive and specific and can be used to accurately diagnose the presence of this bacterium in a sample.