Mycobacterium ulcerans cDNA and Antigen

Mycobacterium ulcerans is the causative agent of Buruli ulcer, an emerging neglected tropical disease that affects skin and soft tissues. This bacterium possesses several antigens and proteins that are believed to play important roles in the pathogenesis of this disease. Let’s take a closer look at some of these antigens:

9.5 kDa culture filtrate antigen Cfp10A: This antigen is a secreted protein of M. ulcerans that is found in the culture filtrate of the bacterium. It has been shown to elicit immune responses and is considered to be an important diagnostic marker for Buruli ulcer.

FbpA 32 kDa surface antigen: FbpA is a surface-exposed antigen of M. ulcerans that is believed to be involved in the adherence of the bacterium to host tissues. It has been shown to be immunogenic and may play a role in the colonization and virulence of M. ulcerans.

Protein antigen 6 Cfp6: Cfp6 is a protein antigen of M. ulcerans that is secreted by the bacterium. It has been shown to stimulate immune responses in infected individuals and is considered to be an important antigen in the context of Buruli ulcer.

T-cell antigen TB8.4: TB8.4 is a T-cell antigen of M. ulcerans that is recognized by the host immune system during infection. It has been shown to elicit immune responses and may play a role in the immune response against M. ulcerans.

Secreted antigen 85-A FbpA and 85-C FbpC: Antigens 85-A (FbpA) and 85-C (FbpC) are two secreted proteins of M. ulcerans that are involved in the lipid metabolism of the bacterium. They have been shown to be important antigens in the context of Buruli ulcer and are believed to play a role in the pathogenesis of the disease.

Mpb51 antigen protein Fbp: Mpb51 is a protein antigen of M. ulcerans that is believed to play a role in the virulence of the bacterium. It has been shown to stimulate immune responses and may be involved in the host-pathogen interaction during M. ulcerans infection.

Soluble secreted antigen Mpt53: Mpt53 is a secreted antigen of M. ulcerans that is believed to be involved in the immune response during Buruli ulcer infection. It has been shown to stimulate immune responses in infected individuals and is considered to be an important antigen in the context of this disease.

These are some of the antigens and proteins of Mycobacterium ulcerans that are believed to play important roles in the pathogenesis of Buruli ulcer. Further research on these antigens and their interactions with the host immune system may contribute to a better understanding of the mechanisms of M. ulcerans infection and the development of improved diagnostic tools and treatment strategies for Buruli ulcer.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Mycobacterium ulcerans cDNA and recombinant antigens are used in the diagnosis of Buruli ulcer, a serious skin infection caused by Mycobacterium ulcerans. Diagnosis of Buruli ulcer is usually based on clinical examination and imaging studies, such as X-ray and ultrasound. However, these methods are not sufficient for accurate diagnosis. Therefore, Mycobacterium ulcerans cDNA and recombinant antigens can be used to detect the presence of the bacteria in clinical samples, such as swab or tissue biopsy. This can help to confirm the diagnosis of Buruli ulcer and facilitate proper treatment. Additionally, these cDNA and recombinant antigens can also be used in epidemiological studies of Buruli ulcer to identify the bacterial strain responsible for the infection and to understand the transmission of the disease.