Pasteurella multocida cDNA and Antigen

Pasteurella multocida is a Gram-negative, facultative anaerobic, coccobacillus-shaped bacterium. It is a component of the normal flora of many animals, including humans, and can cause a variety of illnesses in humans, ranging from mild to severe, including septicemia, pneumonia, meningitis, and other infections. It is found in the respiratory, gastrointestinal, and urogenital tracts of mammals, as well as in the mouths of cats, dogs, and other animals. P. multocida is also a common cause of infections in humans, particularly in cases of animal bites and scratches, or when a person is exposed to an animal’s saliva or other bodily fluids. It is also a cause of food poisoning and can be spread through contaminated food or water. Treatment of P. multocida infections generally involves antibiotics, such as ampicillin, amoxicillin/clavulanate, ciprofloxacin, and tetracycline.In this article, we will discuss the major proteins of Pasteurella multocida and their functions.

Pasteurella multocida 24 kDa Protein
The 24 kDa protein of Pasteurella multocida is a virulence factor that plays a crucial role in the colonization of the bacterium in the host’s respiratory tract. This protein has been shown to induce immune responses in animals, and it is believed to be a potential vaccine candidate.

Pasteurella multocida 47 kDa Protein
The 47 kDa protein of Pasteurella multocida is another virulence factor that is involved in the bacterium’s adhesion to the host cells. This protein has been shown to induce protective immune responses in animals, and it is considered a potential vaccine candidate.

Heat-shock Protein 60 kDa
The heat-shock protein 60 kDa of Pasteurella multocida is a chaperone protein that plays a crucial role in the bacterium’s survival under stress conditions. This protein is essential for the pathogen’s intracellular survival and its resistance to host defenses.

Lipoprotein E
The lipoprotein E of Pasteurella multocida is a surface protein that is involved in the bacterium’s adhesion to the host cells. This protein has been shown to induce immune responses in animals, and it is considered a potential vaccine candidate.

Major MOMP
The major MOMP (major outer membrane protein) of Pasteurella multocida is a porin protein that is involved in the transport of molecules across the bacterial outer membrane. This protein has been shown to induce immune responses in animals, and it is considered a potential vaccine candidate.

OmpA
The OmpA (outer membrane protein A) of Pasteurella multocida is a surface protein that is involved in the bacterium’s adhesion to the host cells. This protein has been shown to induce immune responses in animals, and it is considered a potential vaccine candidate.

Antigen Oma87
The antigen Oma87 of Pasteurella multocida is a major outer membrane protein that is involved in the bacterium’s adhesion to the host cells. This protein has been shown to induce protective immune responses in animals, and it is considered a potential vaccine candidate.

Pasteurella multocida p25
The p25 protein of Pasteurella multocida is a surface protein that is involved in the bacterium’s adhesion to the host cells. This protein has been shown to induce immune responses in animals, and it is considered a potential vaccine candidate.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The cDNA (complementary DNA) and recombinant antigen of P. multocida can be used in various applications for diagnosis, research, and vaccine development.

Diagnostic Tests: cDNA of P. multocida can be used in molecular diagnostic tests to detect the presence of the bacterium in a patient’s sample. This can be done by amplifying a specific genetic target using polymerase chain reaction (PCR) and detecting the amplified product using fluorescence or other methods.

Research: cDNA of P. multocida can be used in research studies to investigate the genetic characteristics and pathogenesis of the bacterium. Recombinant antigens can also be used to study the immune response to P. multocida infections, to identify potential vaccine candidates, and to develop new diagnostic tests.

Vaccine Development: Recombinant antigens of P. multocida can be used to develop vaccines against the bacterium. These vaccines can stimulate the production of specific antibodies that recognize and neutralize P. multocida.