Enterococcus casseliflavus cDNA and Antigen

Enterococcus casseliflavus is a Gram-positive bacterium that belongs to the Enterococcus genus, and it is commonly found in soil, water, and various animal reservoirs. Although it is usually considered a low-virulence organism, it has been reported to cause infections in humans, especially in immunocompromised patients.

Several virulence factors have been identified in Enterococcus casseliflavus, including:

SagBb: SagBb is a secreted antigen that has been shown to induce a significant antibody response in infected patients. It has been proposed as a potential diagnostic marker for E. casseliflavus infections.

60 kDa chaperonin: This protein is a molecular chaperone that helps with protein folding and stabilization. It has been identified as an immunodominant antigen in E. casseliflavus infections and has been proposed as a potential vaccine candidate.

VanC2-3 Ligase: This protein is involved in the synthesis of peptidoglycan and has been shown to confer resistance to vancomycin in E. casseliflavus strains. It is one of the mechanisms of vancomycin resistance observed in Enterococcus species.

EC10 cell surface protein: This protein is a cell surface adhesin that has been shown to be involved in bacterial attachment to host cells. It has been proposed as a potential target for novel antimicrobial therapies.

EC30 collagen adhesin: This protein is also a cell surface adhesin that mediates bacterial attachment to extracellular matrix components such as collagen. It has been shown to be important for bacterial virulence in animal models of infection.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Identification and characterization of virulence factors: The cDNA of E. casseliflavus can be used to study the expression of genes involved in virulence and antibiotic resistance. This information can aid in the development of new treatments for infections caused by this bacterium.

Diagnostic tests: Recombinant antigens of E. casseliflavus can be used as target antigens in diagnostic tests such as ELISA (Enzyme-linked Immunosorbent Assay) for the detection of infections caused by this bacterium.

Antibiotic resistance studies: The cDNA of E. casseliflavus can be used to study the expression of genes involved in antibiotic resistance, which can help in the development of new antibiotics to combat antibiotic-resistant strains of this bacterium.

Probiotic research: E. casseliflavus is a species of enterococcus that is being researched as a potential probiotic, due to its ability to produce lactic acid and its antibacterial properties. The cDNA and recombinant antigen of E. casseliflavus can be used to study its probiotic properties, including its ability to colonize the gut and compete with pathogenic bacteria.