Products

Ehrlichiacanis canis cDNA and Antigen

Cat#	Product Name	Swiss Prot#	Size	Price (US$)	Order
PP0306	Recombinant Protein-Ehrlichiacanis canis 28 kDa major surface antigen-1 (a.a.37 to 287)	Q9ZGJ0	100 µg	1195	Order
PP0307	Recombinant Protein-Ehrlichiacanis canis Immunodominant surface protein gp36 (a.a.20 to 287)	A9XM77	100 µg	1195	Order
PP0308	Recombinant Protein-Ehrlichiacanis canis Major P30-10 (a.a.18 to 280)	Q9F476	100 µg	1195	Order
PP0309	Recombinant Protein-Ehrlichiacanis canis Major P30 (P28-8) (a.a.20 to 288)	Q9ZGJ2	100 µg	1195	Order
PP0310	Recombinant Protein-Ehrlichiacanis canis Major P30-1 (a.a.31 to 307)	Q9ZGJ1	100 µg	1195	Order
PP0311	Recombinant Protein-Ehrlichiacanis canis Major P30-11 (a.a.30 to 279)	Q9ADV8	100 µg	1195	Order
PP0312	Recombinant Protein-Ehrlichiacanis canis Major P30-13 (a.a.46 to 278)	Q9ADW1	100 µg	1195	Order
PP0313	Recombinant Protein-Ehrlichiacanis canis Major P30-14 (a.a.20 to 289)	Q9ADW2	100 µg	1195	Order
PP0314	Recombinant Protein-Ehrlichiacanis canis Major P30-17 (a.a.18 to 316)	Q9ADW5	100 µg	1195	Order
PP0315	Recombinant Protein-Ehrlichiacanis canis Major P30-19 (a.a.37 to 296)	Q9ADW8	100 µg	1195	Order
PP0316	Recombinant Protein-Ehrlichiacanis canis Major P30-20 (a.a.33 to 275)	Q3YQQ6	100 µg	1195	Order
PP0317	Recombinant Protein-Ehrlichiacanis canis Major P30-5 (P28-1) (a.a.25 to 293)	Q9F477	100 µg	1195	Order
PP0318	Recombinant Protein-Ehrlichiacanis canis Major P30-6 (a.a.23 to 294)	Q9ADV4	100 µg	1195	Order
PP0319	Recombinant Protein-Ehrlichiacanis canis Major P30-8 (a.a.21 to 299)	Q9ADV6	100 µg	1195	Order
PP0320	Recombinant Protein-Ehrlichiacanis canis P28-9 (a.a.30 to 271)	Q9F471	100 µg	1195	Order
PP0321	Recombinant Protein-Ehrlichiacanis canisPhosphoribosylaminoimidazole carboxylase (a.a.36 to 363)	Q9AGZ2	100 µg	1195	Order
PP0322	Recombinant Protein-Ehrlichiacanis canis Preprotein translocase SecA subunit (a.a.14 to 106)	Q9ADU9	100 µg	1195	Order
PP0323	Recombinant Protein-Ehrlichiacanis canis Surface antigen D15 (a.a.18 to 418)	Q3YQW1	100 µg	1195	Order
PP0324	Recombinant Protein-Ehrlichiacanis canis Surface antigen msp4 (a.a.26 to 264)	Q3YQS4	100 µg	1195	Order
PP0325	Recombinant Protein-Ehrlichiacanis canis Surface antigen D15 (a.a.61 to 460)	Q3YQW1	100 µg	1195	Order
RPP0306	cDNA-Ehrlichiacanis canis 28 kDa major surface antigen-1 (a.a.37 to 287)	Q9ZGJ0	2 µg	1250	Order
RPP0307	cDNA-Ehrlichiacanis canis Immunodominant surface protein gp36 (a.a.20 to 287)	A9XM77	2 µg	1335	Order
RPP0308	cDNA-Ehrlichiacanis canis Major P30-10 (a.a.18 to 280)	Q9F476	2 µg	1310	Order
RPP0309	cDNA-Ehrlichiacanis canis Major P30 (P28-8) (a.a.20 to 288)	Q9ZGJ2	2 µg	1340	Order
RPP0310	cDNA-Ehrlichiacanis canis Major P30-1 (a.a.31 to 307)	Q9ZGJ1	2 µg	1380	Order
RPP0311	cDNA-Ehrlichiacanis canis Major P30-11 (a.a.30 to 279)	Q9ADV8	2 µg	1245	Order
RPP0312	cDNA-Ehrlichiacanis canis Major P30-13 (a.a.46 to 278)	Q9ADW1	2 µg	1160	Order
RPP0313	cDNA-Ehrlichiacanis canis Major P30-14 (a.a.20 to 289)	Q9ADW2	2 µg	1345	Order
RPP0314	cDNA-Ehrlichiacanis canis Major P30-17 (a.a.18 to 316)	Q9ADW5	2 µg	1490	Order
RPP0315	cDNA-Ehrlichiacanis canis Major P30-19 (a.a.37 to 296)	Q9ADW8	2 µg	1295	Order
RPP0316	cDNA-Ehrlichiacanis canis Major P30-20 (a.a.33 to 275)	Q3YQQ6	2 µg	1210	Order
RPP0317	cDNA-Ehrlichiacanis canis Major P30-5 (P28-1) (a.a.25 to 293)	Q9F477	2 µg	1340	Order
RPP0318	cDNA-Ehrlichiacanis canis Major P30-6 (a.a.23 to 294)	Q9ADV4	2 µg	1355	Order
RPP0319	cDNA-Ehrlichiacanis canis Major P30-8 (a.a.21 to 299)	Q9ADV6	2 µg	1390	Order
RPP0320	cDNA-Ehrlichiacanis canis P28-9 (a.a.30 to 271)	Q9F471	2 µg	1205	Order
RPP0321	cDNA-Ehrlichiacanis canisPhosphoribosylaminoimidazole carboxylase (a.a.36 to 363)	Q9AGZ2	2 µg	1635	Order
RPP0322	cDNA-Ehrlichiacanis canis Preprotein translocase SecA subunit (a.a.14 to 106)	Q9ADU9	2 µg	800	Order
RPP0323	cDNA-Ehrlichiacanis canis Surface antigen D15 (a.a.18 to 418)	Q3YQW1	2 µg	2000	Order
RPP0324	cDNA-Ehrlichiacanis canis Surface antigen msp4 (a.a.26 to 264)	Q3YQS4	2 µg	1190	Order
RPP0325	cDNA-Ehrlichiacanis canis Surface antigen D15 (a.a.61 to 460)	Q3YQW1	2 µg	1995	Order

Please contact us for a quote on larger sizes …

Ehrlichiacanis canis cDNA and recombinant antigen

Codon-optimized cDNA is cloned into E. coli expression vector with 6x His-tag at N-terminus and ready-to-use for recombinant protein production.
Recombinant protein applications: Western Blot may be used for other applications determined by the user.
Protein Purity: >90%, as determined by SDS-PAGE under reducing conditions.
Protein Activity: N/A
Protein Tag: Contains A 6x histidine tag at N-terminus.
Protein Formulation: Liquid
Source: Produced from E. coli

Ehrlichiacanis canis is a species of gram-negative obligate intracellular bacteria that is the causative agent of canine ehrlichiosis. It is transmitted to dogs by bites from infected brown dog ticks and can cause a variety of symptoms ranging from fever, anemia, and lymphadenopathy, to depression, lameness, and even death. The diagnosis of E. canis infection is typically made by looking for the presence of the organism’s antigen in the blood. This is done through a serologic test, such as the indirect fluorescent antibody test, or the enzyme-linked immunosorbent assay.Several proteins have been identified in Ehrlichiacanis canis, which play a crucial role in the pathogenesis of the disease.

One of the most important proteins is the 28 kDa major surface antigen 1, which is responsible for the attachment of the bacteria to the host cells. This protein is also considered a potential vaccine candidate against ehrlichiosis.

Another significant protein is the immunodominant surface protein gp36, which is involved in the binding of the bacteria to the host cells and helps in the establishment of infection. The major P30 proteins (P30-10, P30, P30-1, P30-11, P30-13, P30-14, P30-17, P30-19, P30-20, P30-5, P30-6, and P30-8) are known to play a role in antigenicity and are potential targets for diagnostic tests.

The lipoprotein antigen P28-9 is involved in the stimulation of the host immune system and may be considered as a potential vaccine candidate. Phosphoribosylaminoimidazole carboxylase, Preprotein translocase SecA subunit, and surface antigen D15 are also potential vaccine candidates against ehrlichiosis.

The surface antigen msp4 is involved in the attachment of the bacteria to host cells and helps in the establishment of the infection. The surface antigen D15 is involved in the survival and replication of the bacteria inside the host cells.

Overall, these proteins play a critical role in the pathogenesis of ehrlichiosis and provide potential targets for the development of vaccines and diagnostic tests against the disease.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Ehrlichiacanis canis cDNA and recombinant antigen can be used in ELISA (Enzyme-Linked ImmunoSorbent Assay) to detect E. canis infection in dogs. Using cDNA and recombinant antigen, researchers can develop an ELISA test that is sensitive and specific for the detection of E. canis antibodies. The ELISA test can be used to test for the presence of E. canis antibodies in canine serum or other bodily fluids. The test will detect antibodies to the E. canis antigen, which will indicate that the dog has been exposed to E. canis and may be infected with the pathogen.

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Ehrlichiacanis canis cDNA and Antigen

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