Brucella suis cDNA and Antigen

Brucella suis is a species of Gram-negative bacteria in the genus Brucella. It is an intracellular pathogen, meaning it can infect and replicate within host cells. B. suis commonly infects pigs, but can also infect other species of livestock, wild animals, and humans. In humans, it can cause brucellosis, a systemic infection that primarily affects the reproductive organs and often results in recurrent fevers, fatigue, and joint pain. B. suis can be transmitted to humans through contact with infected animals or their products, such as raw milk or meat. Treatment typically includes a combination of antibiotics, such as doxycycline, rifampin, and streptomycin.

This pathogen produces a range of important proteins and antigens that are involved in its survival and pathogenesis. Here’s a closer look at some of these key proteins and antigens:

Cpn60 groEL protein: This protein is a molecular chaperone that plays an important role in the folding and assembly of other proteins. Cpn60 groEL has been shown to be involved in Brucella suis virulence and survival within host cells.

Bacterial surface antigen: This is a general term used to describe a range of different antigens that are found on the surface of bacteria. In Brucella suis, these antigens are thought to be involved in the bacterium’s ability to interact with host cells and evade the host immune response.

Glycosyl transferase WboA: This protein is involved in the biosynthesis of the O-antigen, a component of the lipopolysaccharide (LPS) layer of the Brucella suis cell wall. The O-antigen is thought to be an important virulence factor, as it helps the bacterium evade the host immune response.

Omp25: This is a major outer membrane protein of Brucella suis that is highly conserved across different species of Brucella. Omp25 is known to be a potent immunogen and is often used in serodiagnostic tests for brucellosis.

Tyrosine recombinase xerD: This is a membrane-associated protein that plays a key role in the maintenance and segregation of the Brucella suis chromosome during cell division.

Urease subunit beta 2: Urease is an enzyme produced by Brucella suis that is involved in the bacterium’s ability to survive in the acidic environment of the host’s phagolysosome. Urease accomplishes this by neutralizing the acidic pH through hydrolysis of urea. Urease subunit beta 2 is one of the key subunits of this enzyme.

In conclusion, Brucella suis produces a range of important proteins and antigens that are involved in its survival and pathogenesis. These proteins and antigens may have potential applications in the development of serodiagnostic tests and vaccines for brucellosis.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Brucella suis cDNA and recombinant antigen can be used in a variety of applications, including diagnostic assays, vaccine development, and immune monitoring. In diagnostic assays, cDNA can be used to amplify target genes in order to detect the presence of Brucella suis in a sample. Recombinant antigens can be used to detect antibodies against Brucella suis in a sample, which can be used to confirm an infection.

In vaccine development, cDNA can be used to create transgenic animals that express protective antigens from Brucella suis. These animals can then be used to test the effectiveness of various vaccine candidates. Recombinant antigens can also be used in vaccine development, as they can be used to create recombinant proteins that can be used as subunit vaccines.

Finally, cDNA and recombinant antigens can be used to monitor the immune response to Brucella suis. This can be done by testing for antibodies against the antigens in a sample, which can be used to measure the body’s immune response to the infection. Additionally, cDNA can be used to measure the expression of certain genes, which can be used to measure the bodies.