Protein A, G, A/G, and L

BcMag™ Protein A/G Magnetic Beads are high-capacity, high-throughput affinity particles used in antibody purification and immunoprecipitation procedures with manual or robotic magnetic separators. The magnetic microspheres are covalently immobilized with a high density ultrapure (Purity>97%) recombinant protein A/G proteins. Protein A/G Magnetic Beads are utilized for antibody purification from serum, cell culture supernatant, or ascites, and antigen IP/Co-IP from cell or tissue extracts. The procedure for Protein A/G Magnetic Beads has been improved to allow maximum recovery and purity of the recovered antibody or antigen. For antibody purification, the beads are incubated with the antibody solution, after which they magnetically separated from the supernatant. For immunoprecipitation, the beads are added to an antigen-containing sample to which an antibody has been introduced and allowed to incubate to form the antibody-antigen complex. The attached antibodies or antigens are dissociated from the beads using an elution buffer and recovered from the solution manually using a magnetic stand or by using automation instruments.