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Quick mRNA Purification Kit
The Ultimate Solution for Intact Poly(A)+ RNA Extraction
Components
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Shipping conditions: At ambient temperature
Handling And Storage: Store The Kit Components According To The Table Above On Arrival.
Components
BcMag™ Oligo-dT Magnetic Beads
1x Binding / Lysis Buffer
10x Washing Buffer I
10x Washing Buffer II
Elution Buffer
Storage
4°C
4°C
4°C
4°C
4°C
Cat. No. MMS101 (50 Preps)
2 ml
10 ml
5 ml
5 ml
0.5 ml
Cat. No. MMS102 (100 Preps)
4.0 ml
Shipping conditions: At ambient temperature
Handling and Storage: Store the kit components according to the table Above on arrival.
Introduction
Poly(A)+ RNA extraction is a crucial step in many downstream applications, such as qRT-PCR, RNA sequencing, and microarray analysis. However, traditional methods for RNA isolation, such as phenol-chloroform extraction, can be time-consuming, labor-intensive, and hazardous to human health due to the use of organic solvents. Therefore, there is an urgent need for a rapid and efficient method for poly(A)+ RNA extraction that ensures the isolation of intact mRNA molecules.
The BcMag™ Quick mRNA Purification Kit is a novel tool designed to extract intact poly(A)+ RNA from cells and tissue samples. Unlike other methods that use chemical solvents like phenol, this kit uses Oligo-d(T)25 attached to 1µm paramagnetic beads to bind the poly(A)+ RNA directly. This makes it possible to manually process multiple samples or adapt the process for high-throughput automated applications. The use of magnetic separation technology allows for the elution of intact mRNA in small amounts, which means that the poly(A)+ transcripts do not need to precipitate in the eluent. In less than an hour, this method can produce intact poly(A)+ RNA that is fully representative of the original sample’s mRNA population.
Workflow: Simplified and User-Friendly
The purification with magnetic beads is a straightforward and user-friendly process. First, mix the beads with the cell lysates and incubate them with continuous rotation for a sufficient time. The beads remain suspended in the sample solution during mixing, allowing the mRNA to bind to oligo-dT magnetic beads. Ribosomal RNA and tiny RNA molecules (transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA) do not adhere to the beads and are eliminated. After incubation, only polyadenylated RNA species (mRNA) are collected and separated from the sample using a magnet rack. Then the ultrapure mRNAs are eluted and used in downstream applications.
Advantages of BcMag™ Quick mRNA Purification Kit
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High Yield: The kit produces a high yield of poly(A)+ RNA from various sources, such as cultured cells, tissues, and blood.
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Fast and Efficient: The entire process takes less than an hour, making it a fast and efficient method for poly(A)+ RNA extraction.
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Compatible with Automated Systems: The process is adaptable to automated systems, allowing for high-throughput applications.
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Easy-to-Use: The protocol is straightforward and user-friendly, making it accessible to a broad range of researchers.
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Intact mRNA: The BcMag™ Quick mRNA Purification Kit ensures the isolation of intact mRNA molecules, which is crucial for downstream applications such as qRT-PCR and RNA sequencing.
Advantages of BcMag™ Quick mRNA Purification Kit
The purified poly(A)+ RNA obtained from the BcMag™ Quick mRNA Purification Kit can be used in various downstream applications, such as:
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qRT-PCR: The kit provides high-quality RNA for qRT-PCR analysis, which is a widely used technique for gene expression analysis.
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RNA Sequencing: The kit produces high-quality RNA suitable for RNA sequencing, which allows for the identification of novel transcripts and alternative splicing events.
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Microarray Analysis: The kit produces high-quality RNA suitable for microarray analysis, which allows for the simultaneous analysis of the expression of thousands of genes.
General Reference
1.
Ferrier DC, Shaver MP, Hands PJW. Micro- and nano-structure-based oligonucleotide sensors. Biosens Bioelectron. 2015 Jun 15;68:798-810.
2.
Sethi D, Gandhi RP, Kuma P, Gupta KC. Chemical strategies for immobilization of oligonucleotides. Biotechnol J. 2009 Nov;4(11):1513-29.
3.
Zuo P, Ye BC. A novel immobilization strategy using oligonucleotide as linker for small molecule microarrays construction. Biosens Bioelectron. 2008 Jun 15;23(11):1694-700.