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Products
Revolutionary BcMag™ One-Step Blood DNA Purification Kit
Components
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Shipping conditions: At ambient temperature
Handling And Storage: Store The Kit Components According To The Table Above On Arrival.
Components
BcMag™ U-DNA Beads
10x Lysis Buffer (100mM Tris-HCl, PH 9.0)
Proteinase K
DTT
Proteinase K Suspension Buffer
Storage
4°C
4°C
-20°C
-20°C
4°C
Cat. No. AF101 (50 Preps)
2.5 ml
0.6 ml
12.5 mg
15.4 mg
1.0 ml
Cat. No. AF102 (100 Preps)
5.0 ml
1.2 ml
25 mg
30.8 mg
2.0 ml
Shipping conditions: At ambient temperature
Handling and Storage: Store the kit components according to the table Above on arrival.
Introduction
Isolation of DNA from blood and other body fluids in adequate quantities is integral to the clinic and forensic research and analysis. Large-scale clinical point-of-care tests require a quick and efficient DNA isolation technique for a trace amount of blood sample or other body fluids. However, current blood DNA purification protocols suffer drawbacks, including low yield, compromised quality, cost, tedious and time-consuming, toxic organic solvents, and many more. To overcome these drawbacks, we developed a novel revolutionary one-step DNA purification system based on magnetic beads and negative chromatography, which combines DNA extraction with removing all the PCR inhibitors from the samples without performing DNA isolation and purification steps.
BcMag™ One-Step Blood DNA Purification Kit allows rapid and efficient purification of genomic DNA from whole blood, serum, plasma, or other body fluids. The kit uses our unique proprietary magnetic beads to efficiently lyse cells and remove all impurities simultaneously in an aqueous buffer, leaving the DNA untouched. The procedure employs mild lysis conditions, avoiding harsh conditions such as alkaline lysis and toxic chemicals for lysing cells to maintain DNA integrity and the time-consuming cleanup of organic solvent from the sample. Furthermore, the magnetic beads eliminate PCR inhibitors (Fig.1) from samples in a single step without DNA extraction. It increases DNA integrity, boosts nucleic acid yields, and minimizes DNA loss caused by typical DNA purification techniques’ time-consuming “bind-wash-elute” procedure. Following sample lysis, the straightforward one-step purification technique enables simultaneous processing of >96 samples and produces pure DNA in less than 30 minutes. Purified genomic DNA has the highest integrity and can be used in various downstream applications such as qPCR, STR, etc.
The Workflow of BcMag™ One-Step Blood DNA Purification Kit
1.
Add magnetic beads to the sample.
2.
Mix the samples with the magnetic beads and proteinase K and heat to lyse the cells.
3.
Mix by vortexing or pipetting for the beads to capture the PCR inhibitors.
4.
Remove the beads with a magnet.
5.
Aspirate the supernatant containing the pure ready-to-use DNA
Performance
The purified DNA is ready for downstream applications, such as PCR, qPCR, RT-PCR (reverse transcription-polymerase chain reaction), single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, genotyping, next-generation sequencing (NGS, veterinary genotyping, forensics, population studies, etc.
Features and Advantages
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Rapid and efficient purification protocol: without prior DNA isolation for subsequent use in direct workflows, No liquid transfer, and One-tube.
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Ultrafast: Process 96 samples in less than an hour.
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Highest nucleic acids recovery rates: Minimal loss of DNA during extraction.
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Effectively cell lysate cleanup and removes inhibitors: polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca2+, Mg2+, etc.
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Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and organic reagents.
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High throughput: Compatible with many different automated liquid handling systems.