Cleavable Linker: A Promising Strategy for Maintaining Physiological Activity in Affinity Chromatography

Bioclone provides magnetic beads linked with different bioconjugate function groups through a cleavable disulfide linker. The beads can immobilize proteins, peptides, DNA/RNA, or biomolecules. The ligand and target molecule complex can be eluted from the beads using reducing agents such as dithiothreitol (DTT) and 2-mercaptoethanol (2-ME) (Fig.1) after affinity purification. These mild elution conditions offer the efficient isolation of the affinity partner from complex protein mixtures. Moreover, a long and hydrophilic cleavable linker reduces the non-specific binding proteins and is suitable for conjugating small or larger biomolecules without a steric hindrance problem. Disulfide cleavable linkers are stable at physiological pH.