- +1 858 909 0079
- +1 858 909 0057
- [email protected]
- +1 858 909 0079
- [email protected]
Cat. No.
Product Name
Unit Size
Order
Specification
Composition
Magnetic beads grafted with a high density of cleavable carboxyl groups
Number of Beads
~ 1.68 x 109 beads/mg (1μm beads)
~1.47 x 108 beads/mg (2.5μm beads)
Stability
Short Term (<1 hour): pH 4-11; Long-Term: pH 4-10
Temperature: 4°C -140°C; Most organic solvents
Magnetization
~40-45 EMU/g
Type of Magnetization
Superparamagnetic
Formulation
Lyophilized Powder
Functional Group Density
1μm Magnetic Beads
~210 μmole / g of Beads
2.5μm Magnetic Beads
~200 μmole / g of Beads
Storage
Store at 4°C upon receipt. Do not freeze.
BcMag™ Cleavable Carboxyl-Terminated Magnetic Beads are uniform, silica-based magnetic beads coated with a high density of carboxylic acid groups on the surface. The high density of carboxylic acid groups on their surface allows efficiently covalently conjugate primary amine-containing ligands via a stable amide bond. Since the active carboxyl group is linked with the beads through a built-in cleavable disulfide linker (Fig.1), reducing agents such as DTT or β-mercaptoethanol can cleave and separate the target molecule-ligand complex from the beads. The cleavable carboxyl magnetic beads are ideal matrices for conjugating large proteins or small peptides. Moreover, the hydrophilic surface ensures low nonspecific adsorption, excellent dispersion, and easy handling in various buffers.
The unique dry form eliminates the need for solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.
The Beads work perfectly as affinity resin for affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a sample containing the target molecules, then mix, incubate, wash and elute the target molecules.
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Easy to use
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A cleavable built-in disulfide bond allows the ligand-target molecule complex separated from the beads.
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Stable covalent bond with minimal ligand leakage
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Produces reusable immunoaffinity matrix
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Low nonspecific binding
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Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
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Applications: Purification for antibodies, proteins/peptides, DNA/RNA, cell sorting,immunoprecipitation.
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