Swine vesicular disease virus cDNA and Antigen

Swine vesicular disease virus (SVDV) is a virus of the Picornaviridae family. It is the only member of the species Enterovirus C, genus Enterovirus. SVDV is the causative agent of swine vesicular disease (SVD), an animal disease that is characterized by the development of vesicles in the feet, snout and interdigital spaces of pigs. Clinical signs of SVD may include lameness, fever, anorexia, and salivation. SVD is a contagious disease and can be spread between pigs through contact with the virus in the vesicular fluid. The virus is also capable of surviving in the environment and can be spread through contaminated objects, such as shoes and clothing.

Swine vesicular disease virus (SVDV) antigen is a protein found on the surface of SVDV-infected cells. It is a major target for the immune system, and it allows the immune system to recognize and eliminate the virus. Antibodies against SVDV antigen can be detected in the serum of infected pigs, and they can be used to diagnose the disease. Vaccines containing SVDV antigen can be used to protect pigs from infection.

Swine vesicular disease virus (SVDV) is a virus belonging to the genus Enterovirus in the family Picornaviridae. It is an RNA virus and its genome is composed of a single-stranded, positive-sense RNA molecule that is approximately 7.2 kb in size. The genome contains a single open reading frame (ORF) that encodes a single polyprotein that is cleaved by the virus’ own proteases into four structural proteins and seven non-structural proteins. The ORF also encodes an additional protein, VPg, which is involved in the initiation of viral replication.

The Capsid protein VP1, VP2, and VP3 form the outer shell of the virus and are essential for its structure and stability. These proteins are also the targets of the host immune system and play a critical role in the development of effective vaccines against SVDV.

Picornain 2A and 3C are proteases that are essential for viral replication, and they cleave the viral polyprotein to generate mature viral proteins. Protein 2B is an integral membrane protein that forms ion channels and is involved in the release of the virus from infected cells.

Other key proteins of SVDV include non-structural protein 3A, which is involved in the formation of replication complexes, and the 3D polymerase, which is responsible for the replication of the viral RNA. The 2C protein plays a role in the assembly of the viral particles, while the 3B protein is involved in the inhibition of host protein synthesis.

Understanding the function and interaction of these proteins is crucial for the development of effective antiviral drugs and vaccines for SVDV. Advances in the study of SVDV proteins and their interactions with host factors can lead to better diagnostic tools, treatments, and preventive measures for this important disease in the pig industry.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.