Respiratory syncytial virus cDNA and Antigen

Respiratory syncytial virus (RSV) is a virus that causes respiratory infections in humans. It is the most common cause of bronchiolitis (inflammation of the small airways in the lungs) and pneumonia in children under the age of one. RSV typically causes mild, cold-like symptoms, such as runny nose, coughing, sneezing, and fever. However, in some cases, it can cause more serious symptoms, such as wheezing or difficulty breathing. RSV is highly contagious and is spread through direct contact with respiratory secretions, such as saliva and mucus, or through contact with contaminated surfaces. Treatment for RSV typically includes rest, fluids, and over-the-counter medications to relieve symptoms. In more severe cases, antiviral medications may be prescribed.

Respiratory syncytial virus (RSV) is a virus that is a major cause of respiratory illnesses in infants and young children. It can also cause severe respiratory illness in adults. RSV antigen is a protein found on the surface of the virus that can be detected with laboratory tests. Tests that detect RSV antigen are used to diagnose RSV infection, to monitor the effectiveness of treatment, and to track the spread of the virus in a population.

Respiratory syncytial virus (RSV) is a single-stranded, negative-sense, non-segmented RNA virus that belongs to the family Paramyxoviridae. Its genome is approximately 15 kb in length and consists of six genes that encode a range of proteins, including the nucleocapsid (N) protein, the attachment (G) glycoprotein, phosphoprotein (P), matrix (M) protein, fusion (F) protein, and the small hydrophobic (SH) protein. The N, P, and M proteins form the viral ribonucleoprotein complex (vRNP), which is responsible for the replication and transcription of the RSV genome. The other three proteins, G, F, and SH are involved in the attachment and fusion of the virus to host cells.

Respiratory syncytial virus (RSV) is a highly contagious virus that can cause respiratory infections in people of all ages. Here are some key proteins associated with RSV:

Phosphoprotein (P): This protein is involved in the replication and transcription of the virus’s RNA genome. It also plays a role in inhibiting the host’s immune response to the virus.

Attachment glycoprotein (G): The G protein helps the virus attach to and enter host cells. It also interacts with the host’s immune system and can modulate the host’s immune response to the virus.

Fusion glycoprotein F0: The F protein is involved in the fusion of the viral envelope with the host cell membrane, allowing the virus to enter the cell. This protein is a target for many RSV vaccines and therapeutic antibodies.

Nucleoprotein (N): The N protein encapsidates the virus’s RNA genome and plays a critical role in virus replication and transcription. It also interacts with the host’s immune system and can stimulate an immune response to the virus.

Understanding these key proteins is important for developing effective treatments and vaccines for RSV.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.