Peste-des-petits-ruminants virus cDNA and Antigen

Peste-des-petits-ruminants (PPR) virus is a highly contagious virus that affects domestic and wild small ruminants, such as sheep and goats. It is a member of the genus Morbillivirus, which also includes measles virus and rinderpest virus. The virus is primarily transmitted through direct contact with infected animals or their secretions, as well as through contaminated feed or water. The primary symptoms of PPR virus infection include fever, coughing, nasal discharge, diarrhea, and mouth ulcers. The disease can lead to severe weight loss and death, and it is considered to be a significant threat to the livelihoods of small-scale farmers in many parts of the world. There is no specific treatment for PPR virus, but vaccines are available and have been shown to be effective in controlling the spread of the disease.

Peste-des-petits-ruminants (PPR) virus antigen refers to a substance that is specifically recognized and targeted by the immune system as being foreign or harmful. Antigens are typically proteins or other molecules found on the surface of viruses and bacteria, and they trigger an immune response. In the case of PPR virus, the virus surface antigen can be used to develop diagnostic tests for the virus and to potentially design vaccines. By exposing the immune system to a specific antigen, it can learn to recognize and respond to the virus if it encounters it again in the future.

The Peste-des-petits-ruminants (PPR) virus genome is the genetic material of the virus, which contains all the information necessary for the virus to replicate and cause disease. The PPR virus genome is a single-stranded RNA (ribonucleic acid) that is approximately 15 kilobases in length. The genome codes for multiple proteins, including the structural proteins (which form the virus shell), the enzymes (which are involved in replication), and the immune modulatory proteins (which help the virus evade the host immune response). Understanding the PPR virus genome is important for developing diagnostic tests, treatments, and vaccines for the virus. Studying the genome can also provide insights into the evolution and transmission of the virus.

The Fusion glycoprotein F0 is a key protein found in PPRV that is involved in the fusion of the virus with host cells, allowing the virus to enter the cell and initiate infection.

The Nucleocapsid is another important protein found in PPRV that forms the viral capsid and protects the viral genome. It is also involved in the replication and transcription of the viral genome.

The M protein is a matrix protein that plays a critical role in the assembly and release of PPRV particles.

The V protein is a non-structural protein that has been shown to inhibit the host immune response, allowing the virus to evade detection and establish infection.

The Hemagglutinin is a protein found on the surface of PPRV particles that plays a role in the attachment of the virus to host cells.

Understanding the roles of these various PPRV proteins is critical for developing effective treatments and vaccines against the virus. Research in this area is ongoing, with the goal of developing new approaches to prevent and treat this highly contagious and economically devastating disease.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E.coli expression Vector), which are ready for production of the recombinant proteins.