JC polyomavirus cDNA and Antigen

JC polyomavirus (JCV) is a type of human polyomavirus that infects most of the population and is usually benign. However, in immunocompromised individuals, JCV can cause a disease called progressive multifocal leukoencephalopathy (PML), which can lead to serious brain damage and death. JCV is primarily found in the urine and feces of infected individuals and can spread through contaminated food or water. It is important for medical professionals to be aware of the presence of JCV in individuals with weakened immune systems and to monitor for symptoms of PML in these patients.

The JC polyomavirus antigen refers to a protein present on the surface of JC polyomavirus (JCV) particles. The antigen is used in diagnostic tests to detect the presence of JCV in clinical samples. This information can be useful in determining whether an individual is infected with JCV and whether they may be at risk of developing progressive multifocal leukoencephalopathy (PML) if they have a weakened immune system. The presence of JCV antigens can be detected through various methods, including immunofluorescence assays, ELISA, and western blot. These diagnostic tests can help inform treatment decisions for individuals infected with JCV.

The JC polyomavirus (JCV) genome is the complete genetic material of the virus, consisting of circular DNA. The JCV genome is small, approximately 5 kilobases in size, and contains a limited number of genes encoding viral proteins. These viral proteins play important roles in the life cycle of the virus, including replication, regulation of the host cell, and evasion of the host immune system. The genetic information encoded in the JCV genome is critical for understanding the biology of the virus and for developing effective treatments for JCV-associated diseases, such as progressive multifocal leukoencephalopathy (PML). The study of the JCV genome has provided valuable insights into the genetics and evolution of polyomaviruses, which are a group of small, non-enveloped, DNA viruses.

The virus is composed of several proteins, with the VP1 major capsid protein being the most abundant. VP1 forms the outer shell of the virus and is responsible for binding to host cells and facilitating viral entry.

VP2 and VP3 are two additional capsid proteins that are also important for viral assembly and stability. VP2 is found on the inner surface of the capsid, while VP3 is in the core of the virus and is involved in the packaging of the viral genome.

In addition to their structural roles, the JC polyomavirus capsid proteins also play a role in viral replication and pathogenesis. For example, VP1 has been shown to be involved in viral transcription and replication, as well as in host immune evasion. Mutations in VP1 have also been associated with increased virulence and the development of PML.

Overall, understanding the roles and functions of JC polyomavirus capsid proteins, including VP1, VP2, and VP3, is critical for developing effective treatments and vaccines for PML and other JC polyomavirus-related diseases.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.