Infectious pancreatic necrosis virus cDNA and Antigen

Infectious pancreatic necrosis virus (IPNV) is a pathogen that infects a variety of fish species, particularly those in the salmonid family. The virus is known for causing infectious pancreatic necrosis, a disease that can lead to significant losses in the aquaculture industry. The virus contains several key proteins that play important roles in its replication and pathogenesis, including:

VP2: VP2 is a major structural protein of IPNV that forms the outer shell of the virus. It is involved in the assembly of new virus particles and is also responsible for virus entry into host cells.

VP4: VP4 is another structural protein of IPNV that is embedded within the virus shell. It is important for the stability of the virus particle and also plays a role in virus entry into host cells.

Submajor capsid protein VP3: VP3 is a minor structural protein of IPNV that is located within the virus shell. It is involved in the assembly and stability of the virus particle.

Understanding the functions of these key proteins is important for developing effective prevention and control strategies for IPNV infections in aquaculture. Research on IPNV and its proteins also has broader implications for the aquaculture industry, given the significant economic impact of viral pathogens on fish health and production.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.