Bovine coronavirus cDNA and Antigen

Bovine coronavirus (BCoV) is a pathogenic virus that can cause a viral disease in cattle. BCoV is a member of the Coronaviridae family and can cause respiratory and enteric symptoms in cattle, leading to decreased weight gain, decreased milk production, and decreased fertility. BCoV is highly contagious and can spread rapidly through cattle populations, making it important to take preventive measures such as vaccination and biosecurity measures to control BCoV infections in cattle populations.

Bovine coronavirus (BCoV) antigen refers to a substance that is recognized by the immune system as foreign and triggers the production of antibodies. This antigen is found in the BCoV virus and can be used in laboratory tests to detect BCoV infections in cattle. Detection of BCoV antigen in blood or serum samples can help diagnose BCoV infections and monitor the effectiveness of vaccines against the virus.

The genome of Bovine coronavirus (BCoV) is the complete genetic material of the virus. It is composed of a single-stranded RNA (ribonucleic acid) molecule and is approximately 27 kilobases in length. The BCoV genome encodes for several proteins that are involved in virus replication, assembly, and pathogenesis. Understanding the BCoV genome can help in the development of diagnostic tools and vaccines for the control of BCoV infections in cattle. The genome can also be used to study the genetic diversity and evolution of BCoV in different cattle populations.

Spike protein: This protein is a structural protein that is responsible for viral attachment and entry into host cells. It is the major target for neutralizing antibodies.

Membrane protein: This protein is a structural protein that forms the envelope of the virus. It is involved in virus assembly and release.

Hemagglutinin-esterase: This protein is a structural protein that is involved in receptor binding and fusion. It is also involved in the hemagglutination of red blood cells.

Nucleocapsid protein: This protein is a structural protein that encapsidates the viral RNA genome. It is a major target for the immune response.

Non-structural protein: BCoV has several non-structural proteins that are involved in virus replication and transcription. These include proteins such as replicase and helicase.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Bovine coronavirus (BCV) cDNA and recombinant antigen can be used in a variety of applications. BCV cDNA can be used to study the genetic make-up of the virus and to develop improved diagnostics and vaccines. The recombinant antigen can be used to develop serological assays to detect antibodies from animals that have been exposed to the virus. It can also be used to measure the effectiveness of vaccines used to protect against BCV infection. Additionally, the recombinant antigen can be used to develop rapid antigen detection tests for the diagnosis of BCV infection.