Porcine rotavirus cDNA and Antigen

Porcine rotavirus is a type of virus that causes gastrointestinal illness in pigs. The virus belongs to the family Reoviridae and is classified as a member of the genus Rotavirus. The genome of porcine rotavirus is composed of 11 segments of double-stranded RNA, which encode for several key proteins involved in the replication and pathogenesis of the virus.

Antigen refers to a molecule that triggers an immune response in the body. Porcine rotavirus antigen is a protein or other molecule present on the surface of porcine rotavirus that can stimulate an immune response, leading to the production of antibodies against the virus. These antibodies can then help to protect against future infection. The development of recombinant antigens can be useful to produce diagnostic tests, vaccines, and other applications.

The genome of porcine rotavirus is composed of 11 segments of double-stranded RNA (dsRNA) that encode for the virus’s structural and non-structural proteins. The genome size of porcine rotavirus ranges from 30 to 40 kilobases. The virus is composed of several structural proteins, including the outer capsid proteins VP4 and C, and the capsid protein VP6.

The outer capsid proteins VP4 and C are essential for viral entry into host cells. VP4 is a major target for neutralizing antibodies and is involved in the cleavage of the viral spike protein. VP4 is also responsible for the induction of protective immunity against porcine rotavirus.The outer capsid protein C is involved in the stability of the virion and the attachment of the virus to host cells. It is also important for the immune response to the virus, as it is a target for neutralizing antibodies.

The capsid protein VP6 is the most abundant protein in the viral particle and is essential for virus assembly and stability. It is also involved in viral replication and transcription.

Porcine rotavirus infections can lead to significant economic losses in the swine industry. Ongoing research is focused on understanding the roles of these structural proteins in viral pathogenesis and the development of effective treatments and vaccines for porcine rotavirus infections.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.