Porcine epidemic diarrhea virus cDNA and Antigen

Porcine epidemic diarrhea virus (PEDV) is a highly contagious virus that affects pigs and causes severe diarrhea, dehydration, and high mortality rates, especially in young pigs. PEDV was first identified in the 1970s and has since become a significant global concern for the pig industry due to its impact on animal health and production. The virus is transmitted through the ingestion of contaminated feces, and outbreaks can occur rapidly and cause widespread economic losses. There is currently no specific treatment for PEDV infections, and control measures are focused on biosecurity and vaccination. The development of effective vaccines and diagnostic tests is an ongoing priority in the fight against PEDV.

The porcine epidemic diarrhea virus (PEDV) antigen is a molecule found on the surface of the virus that can be used to identify the presence of the virus. Antigens are used in diagnostic tests such as ELISA (Enzyme-linked Immunosorbent Assay) and Western blot to detect PEDV antibodies in serum or feces samples from infected pigs. These tests play an important role in controlling PEDV outbreaks by allowing for rapid and accurate diagnosis. Additionally, PEDV antigens can be used to develop vaccines by triggering the immune system to produce antibodies against the virus. These antibodies provide protection against future infections. The development of effective PEDV antigens is critical for improving animal health and controlling the spread of the virus.

The porcine epidemic diarrhea virus (PEDV) genome is the genetic material that encodes the information necessary for the virus to replicate and cause disease in pigs. The genome of PEDV is a single-stranded RNA molecule that is approximately 27 kilobases in length. The genome contains information necessary for the synthesis of the virus’s structural proteins, which make up its envelope, as well as its non-structural proteins, which are involved in replication and pathogenesis. Studying the PEDV genome provides valuable insights into the biology of the virus and is critical for the development of effective vaccines and diagnostic tests. Additionally, understanding the genetic diversity of PEDV strains can help control the spread of the virus and prevent future outbreaks.

The membrane protein is a critical component of the virus envelope and plays an important role in virus assembly and release.

The nucleocapsid protein is responsible for packaging the viral RNA genome into a helical nucleocapsid structure, which protects the genome during transmission.

The spike protein is involved in the binding of the virus to host cells and the fusion of the virus envelope with host cell membranes, which allows the virus to enter and infect cells.

Understanding the structure and function of these three key proteins is essential for developing effective prevention and treatment strategies for PEDV. Ongoing research in this area aims to identify new targets for intervention and to develop new approaches for controlling the spread of this disease in pig populations.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.