Lumpy skin disease virus cDNA and Antigen

Lumpy skin disease (LSD) is a viral disease of cattle that is caused by the Lumpy skin disease virus (LSDV). The disease is characterized by the development of skin lesions, or lumps, that are usually accompanied by fever, weight loss, and reduced milk production. LSD is highly contagious and can spread rapidly through herds of cattle, causing significant economic losses for farmers and the livestock industry.

The LSDV is a large, double-stranded DNA virus that belongs to the genus Capripoxvirus, which also includes other important animal viruses such as sheeppox and goatpox. The virus is transmitted through direct contact between infected and susceptible animals, as well as through insect vectors, such as ticks.

Lumpy skin disease virus (LSDV) antigen is a substance (usually a protein) present in or produced by the LSDV that triggers an immune response in the host. Antigens can be used in the development of diagnostic tests to detect the presence of the virus in a sample. They can also be used in the development of vaccines and therapeutic agents against the virus. The production of antibodies by the immune system in response to an antigen can recognize and neutralize the virus, preventing it from causing disease. The use of LSDV antigen in diagnostic tests and vaccine development can improve our understanding of the virus and our ability to prevent and treat LSD.

The Lumpy skin disease virus (LSDV) genome is the complete genetic material that codes for the virus’s proteins and other essential components. The genome is a double-stranded DNA genome and encodes several structural and non-structural proteins that are involved in the replication and transcription of the virus. Understanding the LSDV genome and its genetic variability can aid in the development of diagnostic tools, vaccines, and antiviral therapies against the virus. Further research is needed to fully understand the biology and genetics of LSDV and how they contribute to the severity of illness and outcomes in infected cattle.Several key proteins have been identified in LSDV that play important roles in its pathogenesis and replication.

Probable host range protein 2 is a LSDV protein that is involved in viral entry into host cells and is likely responsible for determining the virus’s host range.

The major envelope protein of LSDV is a structural protein that plays a critical role in virus assembly and entry into host cells.

P32 antigen is another structural protein of LSDV that is involved in the virus’s replication and assembly. It is also a major target of the host immune response, making it an important target for the development of vaccines and therapeutics.

Finally, the secreted virulence factor NI is a nonstructural protein of LSDV that plays a critical role in the virus’s pathogenesis by suppressing the host immune response.

Understanding the functions and interactions of these LSDV proteins is essential for the development of effective treatments and vaccines for LSDV. Additionally, because LSDV is closely related to other poxviruses, studying LSDV may provide insights into the pathogenesis of other poxviruses, such as smallpox and monkeypox.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.