Infectious salmon anemia virus cDNA and Antigen

Infectious salmon anemia virus (ISAV) is a virus that causes a contagious disease in Atlantic salmon, primarily in fish farms. It is an Orthomyxoviridae virus, and the only known virus in the family to infect fish. ISAV is responsible for significant economic losses in the aquaculture industry, and it has been found in wild salmon populations in Europe, North America, and South America. The virus causes anemia, respiratory distress, hemorrhaging, and death in infected fish, and it is believed to be highly contagious among salmon populations. Diagnosis of the virus is difficult and can involve laboratory testing of tissue and blood samples. Treatment is usually not possible, though vaccinating fish can provide some protection.

Infectious salmon anemia virus (ISAV) is an RNA virus in the family Orthomyxoviridae. It is a pathogen of salmonids and is considered to be one of the most important diseases of farmed salmonids worldwide. The virus is morphologically similar to influenza virus. ISAV antigens are specific proteins found within the virus that can be used to identify its presence. Antibodies can be produced in response to the antigens. Antibodies are then used to detect the virus through diagnostic assays such as ELISA.

Infectious Salmon Anemia (ISA) is a highly contagious virus that affects the health and welfare of Atlantic salmon. The virus is a single-stranded, positive-sense RNA virus belonging to the Orthomyxoviridae family. It is the causative agent of the infectious salmon anemia disease, a serious disease of salmonids. The ISA virus genome consists of eight segments, ranging in size from 1.6 to 2.8 kilobase pairs each. The segments encode six proteins: two surface glycoproteins (HA and NA) involved in receptor binding and virus entry, two nonstructural proteins (NS1 and NS2) involved in replication and transcription, and two nonstructural proteins (NS1 and NS2) involved in virulence strategies. The genome also contains two regulatory regions: the 5′ UTR and the 3′ UTR which control viral replication and transcription.Several key proteins of ISAV are important for its replication and pathogenesis, including:

p18 kDa protein: This is a major structural protein of ISAV that plays an important role in the assembly of new virus particles.

NP protein: The nucleoprotein of ISAV is responsible for protecting the viral genome and ensuring its proper replication.

p32 kDa protein: This is another structural protein of ISAV that is involved in the assembly of new virus particles.

Matrix protein: The matrix protein of ISAV plays a key role in the assembly of new virus particles and is also important for the stability of the virus.

Non-structural protein 1: NS1 is a critical protein of ISAV that is involved in virus replication and pathogenesis.

Research on these key proteins is important for developing effective control and prevention strategies against ISAV infections in aquaculture and could also have broader implications for understanding viral pathogens in fish and other animals.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.