Infectious hematopoietic necrosis virus cDNA and Antigen

Infectious hematopoietic necrosis virus (IHNV) is a virus that causes a highly contagious and potentially lethal disease in salmonid fish species, such as salmon and trout. The virus infects the fish’s red blood cells and kidneys, leading to symptoms such as anemia, stunted growth, and death. IHNV is a significant threat to aquaculture industries and has caused significant economic losses worldwide. Control and prevention measures, such as proper biosecurity and vaccination, are crucial in reducing the spread and impact of IHNV.

Infectious hematopoietic necrosis virus (IHNV) antigen refers to a protein or substance found in or produced by the IHNV that is capable of triggering an immune response. This antigen can be used in the development of diagnostic tests for IHNV infection or for the production of vaccines to prevent the disease in fish species. The presence of IHNV antigen in a sample can be detected using techniques such as ELISA (enzyme-linked immunosorbent assay) or immunofluorescence. The detection of IHNV antigen is an important tool in monitoring and controlling the spread of the virus in aquaculture populations.

The infectious hematopoietic necrosis virus (IHNV) genome refers to the complete set of genetic material present in the virus. The genome of IHNV is composed of RNA (ribonucleic acid) and is characterized by its unique nucleotide sequence. The study of the IHNV genome is important in understanding the biology of the virus, its pathogenesis, and its evolution over time. The information derived from the IHNV genome can be used to develop better diagnostic tools, vaccines, and control measures for IHNV infection in fish species. Additionally, the analysis of the IHNV genome provides insight into the molecular mechanisms involved in the replication and spread of the virus, which is important for the development of antiviral strategies.

Infectious hematopoietic necrosis virus (IHNV) is a negative-sense RNA virus that primarily infects salmonid fish, causing a deadly disease that can have significant economic impacts on the aquaculture industry. IHNV contains several key proteins that are essential for virus replication and pathogenesis. Two of these proteins are:

Matrix protein: The matrix protein of IHNV is involved in virus assembly and is important for the formation of virus particles. It also plays a role in virus budding and release.

Phosphoprotein: The phosphoprotein is a critical component of the virus RNA-dependent RNA polymerase complex, which is responsible for replicating the viral RNA genome. The phosphoprotein is also involved in regulating the activity of the polymerase.

Understanding the functions and interactions of these IHNV proteins is important for developing effective strategies for the prevention and control of IHNV infections in fish populations. Vaccines have been developed that target these proteins, with the aim of providing protective immunity to fish against IHNV infection. Further research on these proteins may also lead to the development of new antiviral drugs for the control of IHNV in aquaculture settings.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.