Infectious bronchitis virus cDNA and Antigen

Infectious bronchitis virus (IBV) is a highly contagious virus that affects poultry, causing respiratory and reproductive issues. The virus can lead to decreased egg production, decreased egg quality, and even death in severe cases. Control measures include biosecurity, vaccination, and prompt culling of infected birds.

Infectious bronchitis virus (IBV) antigen refers to the substance or molecules present on the surface of the IBV that triggers an immune response in the host organism. Antigens are used in diagnostic tests to detect the presence of the virus, and in vaccine development to provide protection against infection. The specific antigenic properties of the IBV virus can change over time, leading to the emergence of new strains and the need for updated vaccines.

The infectious bronchitis virus (IBV) genome is the complete set of genetic material (DNA or RNA) present in the IBV virus particle. It encodes the genetic information necessary for the virus to replicate and cause disease in poultry. The IBV genome is segmented, with several different RNA segments encoding different viral proteins. Understanding the genetic makeup of IBV is important for studying the evolution of the virus and for developing effective vaccines and diagnostic tests.

Infectious bronchitis virus (IBV) is a coronavirus that primarily infects poultry, causing a highly contagious respiratory disease. IBV contains several key proteins that are critical for virus entry, replication, and pathogenesis. These proteins include:

Matrix protein: This protein is involved in virus assembly and is important for the formation of virus particles.

Spike protein: The spike protein plays a key role in virus entry into host cells by binding to receptors on the surface of the host cell.

Nucleocapsid protein: This protein is involved in protecting the virus genome and is important for virus replication.

Membrane protein: The membrane protein is involved in the assembly and release of virus particles.

Envelope protein: The envelope protein is a key structural component of the virus and is involved in virus entry and assembly.

Spike 2 protein: This protein is a structural protein that is involved in the formation of the viral envelope.

S1 surface glycoprotein: The S1 surface glycoprotein is a subunit of the spike protein that plays a critical role in host receptor recognition and virus entry.

S2 glycoprotein: The S2 glycoprotein is the second subunit of the spike protein and is involved in virus-cell membrane fusion during virus entry.

Understanding the functions and interactions of these IBV proteins is important for developing effective strategies for the prevention and control of IBV infections in poultry. Further research on these proteins may also lead to the development of new vaccines and antiviral drugs for the control of IBV.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.