Human astrovirus cDNA and Antigen

Human astrovirus is a small, non-enveloped RNA virus that is a cause of gastroenteritis in humans. Astroviruses are spherical in shape and are approximately 30 nm in diameter. They belong to the family Astroviridae, which contains several other species of astroviruses that infect a variety of animals.

Human astrovirus infection typically results in acute gastroenteritis, which is characterized by symptoms such as diarrhea, vomiting, abdominal pain, and fever. Although astrovirus infections are generally mild, they can be more severe in young children, elderly individuals, and immunocompromised individuals.

The genome of human astrovirus consists of a single, positive-sense RNA molecule that encodes the viral proteins required for replication and assembly of new virions. The genetic diversity among different astrovirus strains has made it difficult to develop a single, effective vaccine for astrovirus infection.

Human astrovirus antigen refers to any substance that can stimulate an immune response in the human body and trigger the production of antibodies against the human astrovirus. Antigens are usually proteins or carbohydrates that are found on the surface of the virus, and are used by the immune system to recognize and respond to the infection.

In the context of human astrovirus, antigens are used to develop diagnostic tests for the detection of astrovirus infections, as well as to produce vaccines to prevent astrovirus infections.

Human astrovirus is a small, non-enveloped RNA virus that commonly causes gastroenteritis in humans. The virus contains a single-stranded positive-sense RNA genome, and its life cycle is regulated by several key viral proteins, including the capsid protein, immunoreactive viral protein, non-structural polyprotein 1A, p19 protein, and p20 protein.

The capsid protein is a structural protein that forms the outer shell of the virus, protecting the RNA genome from the external environment. It also plays a critical role in viral entry into host cells and the initiation of the viral replication cycle.

The immunoreactive viral protein is a highly antigenic protein that elicits an immune response in infected individuals. It is often used as a diagnostic marker for astrovirus infection.

The non-structural polyprotein 1A is a precursor protein that is cleaved into several smaller proteins, including the RNA-dependent RNA polymerase, which is responsible for replicating the viral genome.

The p19 protein and p20 protein are non-structural proteins that are thought to be involved in regulating viral replication and modulating host immune responses. Research is ongoing to better understand the precise roles of these proteins in the astrovirus life cycle.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E.coli expression Vector), which are ready for production of the recombinant proteins.