Feline infectious peritonitis virus cDNA and Antigen

Feline infectious peritonitis (FIP) is a viral disease caused by the feline coronavirus (FCoV). It is characterized by inflammation of the lining of the abdominal cavity and the formation of fluid-filled pockets called effusions. The virus can also infect other organs such as the lungs, leading to severe respiratory symptoms. FIP is considered a serious and often fatal disease in cats and there is no known cure. However, there are vaccines available to help prevent the disease and early diagnosis and treatment can help manage symptoms.

The Feline infectious peritonitis (FIP) virus genome is the genetic material of the virus, which is composed of RNA. The genome of the FIP virus is made up of a single strand of RNA, which is approximately 15kb in length. The genome encodes for several structural and non-structural proteins that are essential for the replication and pathogenesis of the virus. The genetic information in the genome of the virus is used to identify and diagnose the virus, as well as to understand its biology and the mechanisms of its replication. It is also used in the development of diagnostic tools and vaccines.

Feline infectious peritonitis virus antigen refers to a specific protein or component of the virus that can be used to identify the presence of the virus in a sample. This can be used in diagnostic tests to detect FIP in a cat. Antigens can be used in a variety of diagnostic methods such as ELISA, PCR, and virus neutralization tests. The detection of antigen can be used to confirm an FIP infection, however, other methods such as PCR are more sensitive and specific.

The FIPV virion is composed of several structural proteins, including:

Membrane protein – a protein that forms the outer layer of the virion and is involved in viral assembly and budding.

Nucleocapsid – a protein that encapsulates the viral RNA genome and is involved in viral replication and packaging.

Glycoprotein 7b – a glycosylated protein that is involved in viral entry into host cells.

Glycoprotein 7a – another glycosylated protein that is involved in viral entry into host cells.

These structural proteins work together to allow the virus to infect and replicate within host cells. The glycoproteins 7a and 7b are important for the virus to enter host cells by binding to specific cellular receptors. The membrane protein is involved in the assembly and release of new virus particles. The nucleocapsid protein encapsulates the viral RNA genome and is involved in viral replication and packaging.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.