Cowpox virus cDNA and Antigen

Cowpox virus is a zoonotic virus that affects domesticated animals, particularly cows, and can also cause a mild infection in humans. The virus is closely related to smallpox virus and is a member of the Orthopoxvirus genus. The natural host for cowpox virus is the European wildcat, but domesticated cats can also be infected. The virus is transmitted from animals to humans through direct contact with infected animals or through contaminated objects, such as bedding or feed. Human infection with cowpox virus typically results in a mild skin rash and lesions, which can be treated with antibiotics.

The genome of cowpox virus is a double-stranded DNA molecule that is approximately 130-200 kilobases in length, depending on the strain. It encodes several hundred proteins that are involved in various functions, such as replication, evasion of host immunity, and pathogenesis. The genome of cowpox virus is organized into a series of genes that encode the virus’s structural and non-structural proteins, as well as its regulatory elements. Additionally, the genome of cowpox virus is used as a model system for studying the evolution and diversity of DNA viruses, as well as for developing methods for gene delivery and expression in various host cells.

The antigen of cowpox virus refers to any substance that triggers an immune response in infected individuals. Antigens are typically composed of viral proteins that are present on the surface of the virus or produced during replication. These antigens are recognized by the host’s immune system and can elicit the production of antibodies that neutralize the virus. In cowpox virus infection, the host’s immune response to the viral antigens is important in controlling the spread of the virus and preventing the development of severe symptoms.

Hemagglutinin is a viral protein that plays a role in the attachment of the virus to host cells. The 21kDa protein is not a known protein of Cowpox virus.

Core protein is a term that refers to a group of viral proteins that are found in the viral core and are involved in the packaging and protection of the viral genome.

VETF (Vaccinia virus E3L homologous protein with a thrombospondin type 1 motif) is a protein found in cowpox virus that is involved in the suppression of the host immune response. It has a similar function to the E3L protein found in vaccinia virus.

Major membrane protein is a viral protein that is a component of the viral envelope and is involved in the entry of the virus into host cells.

Secreted protein is a general term that refers to any protein that is secreted by a cell, including viral proteins. Without knowing the specific secreted protein, it’s difficult to provide further information.

Understanding the roles of these key proteins in the life cycle of cowpox virus is important for developing effective strategies to prevent and treat infections. Ongoing research is focused on developing antiviral drugs that target these proteins, as well as improving diagnostic tests and vaccines for the virus. By better understanding the molecular mechanisms underlying cowpox virus infections, we can work towards better managing and controlling this zoonotic disease.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.