- +1 858 909 0079
- +1 858 909 0057
- [email protected]
- +1 858 909 0079
- [email protected]
Cat. No.
Product Name
Unit Size
Order
Specification
Composition
Magnetic beads grafted with tosyl group on the surface
Number of Beads
~ 1.68 x 109 beads/mg (1μm beads)
~ 5 x 107 beads /mg (5μm beads)
Stability
Short Term (<1 hour): pH 4-11; Long-Term: pH 4-10
Temperature: 4°C -140°C; Most organic solvents
Magnetization
~40-45 EMU/g
Formulation
Lyophilized Powder
Functional Group Density
1μm Magnetic Beads
~250 μmole / g of Beads
5μm Magnetic Beads
~210 μmole / g of Beads
1μm Long-Arm Magnetic Beads
~200 μmole / g of Beads
5μm Long-Arm Magnetic Beads
~180 μmole / g of Beads
Storage
Ship at room temperature. Store at -20°C, free of moisture upon receipt.
BcMagTM Tosyl Activated Magnetic Beads are magnetic beads grafted with tosyl functional groups on the surface. Short and long-arm tosyl-activated magnetic beads can efficiently conjugate ligands containing primary amine in either aqueous or organic solvents (30% DMF) without introducing any charge (Fig.1). The Tosyl Activated Magnetic Beads are suitable for conjugation of large size protein, while long-arm tosyl activated magnetic beads are ideal for coupling small molecules without steric hindrance problem.
The Tosyl Activated magnetic resins are an ideal choice for covalently attaching antibodies, peptides, complete proteins, and functional enzymes to the surface. The immobilized beads are widely used in Immunoprecipitation of proteins and protein complexes due to their low background and covalent binding of antibodies to the bead surface.
The Tosyl Activated magnetic resins coupling reaction is carried out at 37°C, and pH ranges from neutral to high. We advocate coupling at pH 8.5-9.5, but coupling with pH labile ligands can be done in an alternate buffer at pH 7.4.
The unique dry form eliminates the need for acetone solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.
The tosyl-activated magnetic beads work perfectly as solid support for various bioseparations to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a solution containing the target molecules, then mix, incubate, wash and elute the target molecules.
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Pre-activated and ready-to-use
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Easy to use
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No charge remains on the surface after coupling
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Stable covalent bond with minimal ligand leakage
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Produces reusable immunoaffinity matrices
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Low nonspecific binding
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Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
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Applications: Cell sorting, Immunoprecipitation, purification for antibodies, proteins/peptides, DNA/RNA
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