- +1 858 909 0079
- +1 858 909 0057
- [email protected]
- +1 858 909 0079
- [email protected]
Cat. No.
Product Name
Unit Size
Order
Specification
Composition
Magnetic beads imobilized with IDA group on the surface
Number of Beads
~ 1.68 x 109 beads/mg (1μm beads)
~ 5 x 107 beads /mg (5μm beads)
Stability
Short Term (<1 hour): pH 3-11; Long-Term: pH 4-10
Temperature: 4°C -140°C; Most organic solvents
Magnetization
~40-45 EMU/g
Type of Magnetization
Superparamagnetic
Functional Group Density
1μm Magnetic Beads
~40 μMol NiSO4 / Gram of Beads
5μm Magnetic Beads
~32 μMol NiSO4 / Gram of Beads
Storage
Store at 4°C upon receipt. Do not freeze.
BcMag™ IDA Magnetic Beads are 1μm and 5μm in diameter and highly uniform magnetic microspheres covalently immobilized with a high density of IDA (Iminodiacetic Acid) ligand designed for capture and purification of proteins from various sample types. The microspheres combine all the advantages of Immobilized metal ion affinity chromatography (IMAC) protein purification such phosphoproteins, phosphopeptides or his-tagged protein (low costs, simplicity, high specificity, and capacity) and magnetic properties to perform efficient manual or automatic quick high-throughput purification.
BcMag™ IDA Magnetic Beads work perfectly as solid resin for various affinity purifications to refine molecules, cells, and parts of cells into purified fractions. Add the beads to a solution containing the target molecules, then mix, incubate, wash, and elute the target molecules
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Easy to use
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More efficient and low nonspecific binding
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Stable covalent bond with minimal ligand leakage
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Produces reusable matrices
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Low nonspecific binding
When selecting ligands for IMAC, NTA and IDA are the two most used options. NTA has a coordination number of 4 and is a tetravalent ligand, resulting in strong coordination of metal ions. In contrast, IDA has a coordination number of 3 and is a trivalent ligand. NTA typically provides higher binding specificity and lower metal ion leaching, while IDA has more non-specific binding and greater metal ion leaching. IDA can handle higher metal ion loading density and often requires a lower imidazole concentration in the eluent.
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Fax: +1-858-909-0057
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