Products

IDA-His-tagged Protein Purification Kit

Products

BcMag™ IDA-Ni His-tagged Protein Purification Kit
Cat. No.  MHN101

Unit Size  5 ml
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BcMag™ IDA-Ni His-tagged Protein Purification Kit
Cat. No.  MHN102

Unit Size  10 ml
Order
BcMag™ IDA-Ni His-tagged Protein Purification Kit
Cat. No.  MHN103

Unit Size  50 ml
Order
BcMag™ IDA-Co His-tagged Protein Purification Kit
Cat. No.  MHO101

Unit Size  5 ml
Order
BcMag™ IDA-Co His-tagged Protein Purification Kit
Cat. No.  MHO102

Unit Size  10 ml
Order
BcMag™ IDA-Co His-tagged Protein Purification Kit
Cat. No.  MHO103

Unit Size  50 ml
Order
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Cat. No.

Product Name

Unit Size

Order

MHN101

BcMag™ IDA-Ni His-tagged Protein Purification Kit

5 ml

MHN102

BcMag™ IDA-Ni His-tagged Protein Purification Kit

10 ml

MHN103

BcMag™ IDA-Ni His-tagged Protein Purification Kit

50 ml

MHO101

BcMag™ IDA-Co His-tagged Protein Purification Kit

5 ml

MHO102

BcMag™ IDA-Co His-tagged Protein Purification Kit

10 ml

MHO103

BcMag™ IDA-Co His-tagged Protein Purification Kit

50 ml

Specification

Composition

Magnetic microsphere charged with Ni2+ or Co2+

Magnetization

~60 EMU/g

Type of Magnetization

Superparamagnetic

Effective Density

2.5 g/ml

Concentration

100 mg/ml (1% NiSO4.6H2O or 1% CoCl26H2O)

Binding Capacity

>2mg His-tagged GFP /ml of Beads

Storage

Store at 4ºC upon receipt

Shipping conditions: At ambient temperature

Handling and Storage: Store the kit at 4ºC on arrival.

Description

Introduction

BcMag™ IDA Magnetic Beads are 5µm in diameter and highly uniform IMAC magnetic microspheres covalently immobilized with a high density of IDA (iminodiacetate) ligand. It is designed for capture and purification of histidine-tagged proteins from various sample types. The microspheres combine all the advantages of IMAC protein purification (low costs, simplicity, high specificity, and capacity) and magnetic properties to perform efficient manual or automatic quick high-throughput purification. Bioclone offers two ion-charged IDA magnetic beads for His tag protein purification; BcMag™ IDA-Ni2+ magnetic beads and BcMag™ IDA-Co2+ magnetic beads. The most used metal ion is nickel (Ni2+) for poly (His) fusion proteins since it gives a high yield, whereas the cobalt ion (Co2+) can provide higher purity but with a lower yield.

Workflow

The purification with magnetic microparticles is straightforward. Mix the microparticles with the cell lysate and incubate them with continuous rotation for a sufficient time. During mixing, the beads remain suspended in the sample solution, allowing the target molecules to interact with the immobilized ligand. After incubation, the beads are collected and separated from the sample using a magnet rack. Then the ultrapure His-tagged recombinant proteins are eluted by imidazole.

Workflow of IDA His-tagged protein purification

Features and Advantages

Quick, Easy, and one-step high-throughput procedure; eliminates columns or filters or a laborious repeat of pipetting or centrifugation.

Stable covalent bond with minimal ligand leakage

High binding capacity, very low nonspecific binding

Scalable – Easily adjusts for sample size and automation

Reproducible results

Applications

Investigating protein structure and function

Preparing recombinant proteins for X-ray crystallography

Ideal for study of protein interactions with protein or DNA

Immunization to raise antibodies against a protein of interest

Effective screening protein expression even with crude cell lysates

Microscale purification of his-tagged proteins.

Background information

A polyhistidine tag, also called 6xHis-tag, His-tagged, and His-tag, is a versatile tool for purifying the highly purified recombinant protein from various expression systems, including bacterial, yeast, plant cell, and mammalian cells systems. The tag comprises six or more consecutive histidine amino acid residues positioned at either N or C terminus of a recombinant protein. Due to its small size, His-tag has several distinctive features, including less immunogenicity, hydrophilic nature, and versatility under native and denaturing conditions. Additionally, it is unnecessary to cleave the tag from the recombinant protein since it rarely perturbs the structure and function of its fusion protein. The purification principle of the His-tag depends on immobilized metal ion affinity chromatography (IMAC).

Immobilized metal ion affinity chromatography (IMAC) is a rapid affinity purification chromatography where the his-tagged protein are separated based on their affinity for Ni2+ or Co2+that have been immobilized by a chelator to a solid matrix such as beaded agarose or column. At pH 7-8, his tagged protein will bind to Ni2+ or Co2+. The binding reaction with the tagged protein is affected by various independent variable factors such as pH, temperature, salt type, salt concentration, immobilized metal and ligand density, and protein size. The bound protein is eluted by a decreasing pH gradient, increasing imidazole concentration, or adding an EDTA chelator in a buffer. This technique is an ideal tool for capturing and purification of his-tagged recombinant protein in a quick, inexpensive, and straightforward manner.

Although IMAC is a very effective protein purification technique, they are mainly based on traditional affinity chromatography matrices such as agarose resin or column. These solid matrices make the purification process tedious, time-consuming, unable to handle very tiny samples, and challenging to adapt to the automation system. We developed an extremely efficient magnetic IMAC separation system to overcome these limitations.

General Reference

1.

Jansen, J-C. (Editor). (2011). Protein Purification: Principles, High-Resolution Methods, and Applications. 3rd edition. Volume 151 of Methods of Biochemical Analysis. John Wiley & Sons, Hoboken, NJ

2.

Bornhorst, J.A. and Falke, J.J. (2000). Purification of Proteins Using Polyhistidine Affinity Tags. Methods Enzymol. 326: 245-254.

3.

Spriestersbach A, Kubicek J, Schäfer F, Block H, Maertens B. Purification of His-Tagged Proteins. Methods Enzymol. 2015;559:1-15. doi: 10.1016/bs.mie.2014.11.003. Epub 2015 May 4. PMID: 26096499.

4.

Zhang C, Fredericks D, Longford D, Campi E, Sawford T, Hearn MT. Changed loading conditions and lysate composition improve the purity of tagged recombinant proteins with tacn-based IMAC adsorbents. Biotechnol J. 2015 Mar;10(3):480-9. doi: 10.1002/biot.201400463. Epub 2014 Oct 31. PMID: 25303209.

5.

Pina AS, et al. (2014) Affinity tags in protein purification and peptide enrichment: An overview. Methods in molecular biology (Clifton, N.J.) 1129: 147-168.

6.

Young CL, et al. (2012) Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications. Biotechnol J 7(5): 620-634.

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