Hydrazide-Terminated Magnetic Beads

BcMag™ Hydrazide-Terminated Magnetic Beads are uniform magnetic beads grafted with a high density of hydrazide functional groups on the surface. Hydrazide chemistry is effective for labeling, immobilizing, or conjugating glycoproteins via glycosylation sites, which are frequently (as with most polyclonal antibodies) positioned in domains distant from the critical binding sites whose function needs to be preserved. Coupling antibodies in this manner selectively targets heavy chains in the Fc portion of the molecule, assisting in the best possible preservation of antigen binding activity by the ends of the Fv regions. At pH 5 to 7, hydrazide-terminated supports and compounds will conjugate with carbonyls of oxidized carbohydrates (sugars), resulting in the production of hydrazone linkages. The hydrazide beads are suitable for conjugating larger glycoproteins and glycolipids, carbohydrates, or other ligands. Moreover, the hydrophilic surface ensures low nonspecific adsorption, excellent dispersion, and easy handling in various buffers.