- +1 858 909 0079
- +1 858 909 0057
- [email protected]
- +1 858 909 0079
- [email protected]
Cat. No.
Product Name
Unit Size
Order
FC109
BcMag™ Epoxy-Activated Magnetic Beads Conjugation Buffer Kit
Kit Components
– 10x coupling Buffer: 25 ml 1M Sodium carbonate buffer, pH 7.0
– Blocking Buffer: 25 ml 1 M Ethanolamine, pH 9
– 10 x Wash Buffer: 24 ml 10x PBS, pH 7.2
Each
Specification
Composition
Magnetic beads are grafted with a high density of epoxy groups
Number of Beads
~ 1.68 x 109 beads/mg (1μm beads)
~ 5 x 107 beads /mg (5μm beads)
Stability
Short Term (<1 hour): pH 3-11; Long-Term: pH 4-10
Temperature: 4°C -140°C; Most organic solvents
Magnetization
~40-45 EMU/g
Type of Magnetization
Superparamagnetic
Formulation
Lyophilized Powder
Functional Group Density
1μm Magnetic Beads
~250 μmole / g of Beads
5μm Magnetic Beads
~195 μmole / g of Beads
1μm Long-Arm Magnetic Beads
~200 μmole / g of Beads
5μm Long-Arm Magnetic Beads
~172 μmole / g of Beads
Storage
Ship at room temperature. Store 4°C upon receipt.
BcMag™ Epoxy-Activated Magnetic Beads are pre-activated, uniform magnetic beads coated with high-density epoxy functional groups on the surface. The beads can covalently conjugate amine, sulfhydryl, or hydroxyl group-containing ligands (Fig.1). Moreover, the hydrophilic surface ensures beads have low nonspecific adsorption, excellent dispersion, and easy handling in various buffers. The epoxy-activated magnetic beads are most suitable for the conjugation of large proteins. BcMag™ Long-arm Epoxy-Activated Magnetic Beads are recommended to conjugate small peptides because the long-arm hydrophilic linker may reduce steric hindrance.
Any ligands such as antibodies, peptides, complete proteins, and functional enzymes can be covalently linked to the bead’s surface in a simple overnight reaction. Incubate the ligand you intend to use for affinity purification with these epoxy-activated beads at high pH (8.5-9.5) and 37°C. Coupling can be done in an alternate buffer at pH 7.4 for pH labile ligands.
The unique dry form eliminates the need for acetone solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.
BcMag™ Epoxy-Activated Magnetic Beads work perfectly as affinity resin for affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a sample containing the target molecules, then mix, incubate, wash and elute the target molecules.
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Pre-activated and ready-to-use
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Stable covalent bond with minimal ligand leakage
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Produces reusable immunoaffinity matrices
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Low nonspecific binding
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Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
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Applications: Cell sorting, immunoprecipitation, purification for antibodies, proteins/peptides, DNA/RNA
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