- +1 858 909 0079
- +1 858 909 0057
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- +1 858 909 0079
- [email protected]
Specification
Composition
Magnetic beads grafted with cyanopropyl groups
Number of Beads
~ 1.68 x 109 beads/mg (1μm beads)
~1.47 x 108 beads/mg (2.5μm beads)
Magnetization
~45 EMU/g
Type of Magnetization
Superparamagnetic
Effective Density
2.0 g/ml
Stability
Most organic solvents
Formulation
Lyophilized Powder
Binding Capacity
1 μm beads: >20 μg protein/mg of Beads
2.5 μm beads >18 μg protein/mg of Beads
Storage
Store at 4°C upon receipt.
BcMag™ Cyanopropyl Magnetic Beads are uniform superparamagnetic resins containing cyanopropyl groups on their surface. The beads are specifically designed for quickly purifying, desalting, and concentrating femtomolar to the picomolar scale of very hydrophobic peptides or proteins that may be irreversibly retained on more hydrophobic C18 beads, manually or automatically without the need for a laborious repeat of pipetting and centrifugation.
Magnetic cyanopropyl (CN) bead is a mildly hydrophobic resin that can be used as a normal or reverse phase adsorbent. Under normal phase conditions, the polar component is removed from the non-polar organic solution. Under reversed-phase conditions, non-polar or weakly polar acid, medium, and basic biomolecules are removed from the aqueous solution. Furthermore, as a complex ligand, the cyanopropyl group can concentrate certain metal ions in an aqueous solution.
Hydrophobic interaction chromatography (HIC) is a technique for separating macromolecules from one another based on a reversible interaction between the external hydrophobic region of a biological macromolecule and the hydrophobic ligand (such as phenyl, octyl, or butyl) of a HIC medium. The interaction is enhanced by a buffer with a high salt concentration and reduced with a low salt concentration. Therefore, based on salt concentration in a buffer, the protein with less hydrophobicity is eluted first, whereas the protein with more hydrophobicity elutes last. Compared with other chromatography methods, HIC is a more popular method for separating and purifying protein and peptides in analytical and preparatory scale applications since it employs a less denaturing environment.
Bioclone hydrophobic magnetic resins are designed as uniform magnetic beads grafted with a high density of hydrophobic ligands on the surface. The hydrophobic magnetic beads are rigid polymeric beads with covalent surface chemistries, allowing easier handling and packing while providing more excellent physical and chemical stability—resulting in a robust production process. The beads replace time-consuming, difficult, and expensive chromatographic techniques such as agarose, cellulose, Sepharose, Sephadex-based columns, or resins. The hydrophobic magnetic beads are manufactured using nanometer-scale superparamagnetic iron oxide as core and entirely encapsulated by a high purity silica shell, ensuring no leaching problems with the iron oxide. The pure inert silica makes less nonspecific binding. The beads are much smaller (1 and 5 µm diameter) in size and are non-porous, which exhibit larger surface area, less nonspecific binding, and higher resolution than porous supports.
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