Products

Azide-Activated Magnetic Beads for Biomolecule Enrichment

Products

1 μm BcMag™ Azide-Activated Magnetic Beads
Cat. No.  IM101

Unit Size  150 mg
Order
1 μm BcMag™ Azide-Activated Magnetic Beads
Cat. No.  IM102

Unit Size  300 mg
Order
5 μm BcMag™ Azide-Activated Magnetic Beads
Cat. No.  IM103

Unit Size  150 mg
Order
5 μm BcMag™ Azide-Activated Magnetic Beads
Cat. No.  IM104

Unit Size  300 mg
Order
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Cat. No.

Product Name

Unit Size

Order

IM101

1 μm BcMag™ Azide-Activated Magnetic Beads

150 mg

IM102

1 μm BcMag™ Azide-Activated Magnetic Beads

300 mg

IM103

5 μm BcMag™ Azide-Activated Magnetic Beads

150 mg

IM104

5 μm BcMag™ Azide-Activated Magnetic Beads

300 mg

Specification

Composition

Magnetic beads grafted with a high density of azide groups on the surface

Number of Beads

~ 1.68 x 109 beads/mg (1μm beads)

~ 5 x 107 beads /mg (5μm beads)

Stability

Short Term (<1 hour): pH 4-11; Long-Term: pH 4-10

Temperature: 4°C -140°C; Most organic solvents

Magnetization

~40-45 EMU/g

Type of Magnetization

Superparamagnetic

Formulation

Lyophilized Powder

Functional Group Density

1μm Magnetic Beads

~40 nmole / mg of Beads

5μm Magnetic Beads

~35 nmole /mg of Beads

Storage

Ship at room temperature, Store at 4°C upon receipt.

Description

BcMag™ Azide-Activated Magnetic Beads are uniform inert silica-enclosed magnetic beads grafted with a high density of azide functional groups on the surface (Fig.1). The beads efficiently enrich alkyne-tagged biomolecules from complex cell lysates via a Cu(I)-catalyzed Alkyne-Azide (CUAAC) reaction. Compared with other affinity resins such as agarose or other polymers beads, the inert silica enclosed magnetic beads offer high stability, low nonspecific binding, and superior handling in protein-based systems. These magnetic beads are an ideal matrix for genomics, proteomics, biomarker discovery, posttranslational modification (PTM) analysis, etc.

Structure of Azide-activated Magnetic Beads

Workflow

The beads work perfectly as affinity resin for capturing azide-tagged biomolecules from complex cell lysate. Add the beads to a sample containing the tagged biomolecules, then mix, incubate, wash and elute the target molecules.

Workflow of magnetic beads for affinity purification

Features and Advantages

Easy to use

More efficient and low nonspecific binding

Stable covalent bond with minimal ligand leakage

Produces reusable matrices

Low nonspecific binding

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