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Products
Bone and Teeth DNA Purification Kit
Components
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Shipping conditions: At ambient temperature
Handling And Storage: Store The Kit Components According To The Table Above On Arrival.
Components
BcMag™ HO-DNA Beads
1x Lysis Buffer
1x Elution Buffer
Proteinase K
Proteinase K Suspension Buffer
DTT
Storage
4°C
4°C
4°C
-20°C
4°C
-20°C
Cat. No. AB101 (50 Preps)
0.75 ml
5.0 ml
1.5 ml
10 mg
0.5 ml
77 mg
Cat. No. AB102 (100 Preps)
1.5 ml
10 ml
3.0 ml
20 mg
1.0 ml
154 mg
Shipping conditions: At ambient temperature
Handling and Storage: Store the kit components according to the table Above on arrival.
Introduction
Bones and teeth are frequently the only DNA samples for identifying human remains. The purification of DNA from bones and teeth is important in many molecular biology applications, including forensic analysis, ancient DNA analysis, and genetic studies. The effective extraction of DNA from these tough and hard-to-liquefy samples, such as bones and teeth, is significantly more difficult than analyzing fresh tissues. Bone is a highly organized, sophisticated, and specialized connective tissue with significant calcium levels. The majority of the DNA in bone is found in osteocytes. Because hard connective tissue has a high calcium content, DNA in bones and teeth is often better conserved than in soft tissues. Enamel, dentin, cementum, and pulp tissue make up teeth. Dentine and pulp both contain DNA. Because of the extensive mineralization, selecting an efficient DNA extraction process is critical to eliminate PCR inhibitors and avoid excessive sampling quantities of minerals.
The ability to extract ancient DNA sequences and short tandem repeat (STR) data from bones and teeth exposed to various environmental conditions over time has become a powerful tool for identifying missing people and unknown remains. Due to mineralization, low amounts of endogenous DNA, environmental, bacterial, and postmortem DNA damage, as well as the existence of environment-born inhibitors that co-extract with DNA, recovering DNA data from damaged specimens can still be challenging. Despite the availability of commercial technologies and procedures, bone extraction for forensic operations offers problems. Analysts struggle with low template DNA, significant degradation, and generally suppressed genetic material, which limits STR profile development for identifying reasons. Because of the above-mentioned considerable factors, selecting an efficient DNA extraction process is critical to minimizing mineral sampling and removing polymerase chain reaction (PCR) inhibitors.
BcMag™ Bone and Teeth DNA Purification Kit is designed to extract total nucleic acids from bone and teeth samples efficiently and sequentially. The kit uses our unique proprietary magnetic beads in combination with an optimized demineralization buffer to isolate higher yield and super quality of DNA. Purified genomic DNA has the highest integrity and can be used in various downstream applications such as qPCR, STR, etc. The procedure employs mild lysis conditions, avoiding harsh conditions such as alkaline lysis and toxic chemicals for lysing cells to maintain DNA integrity and the time-consuming cleanup of organic solvent from the sample.
Workflow of Bone-Teeth DNA Isolation
1.
Add lysis buffer and proteinase K to the sample to lyse the bone and incubate at 65°C.
2.
Add functional magnetic beads and vortex/pipette the beads with the sample to the DNA.
3.
Wash the beads.
4.
Separate the beads from the sample using a magnet.
5.
Elute DNA from the beads.
Bone and Teeth DNA Purification Kits are used in a variety of molecular biology applications, including:
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Forensic analysis: DNA extracted from bones and teeth is commonly used in forensic analysis to identify individuals and solve crimes.
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Ancient DNA analysis: DNA extracted from bones and teeth can be used to study the genetics of ancient populations and species.
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Genetic studies: DNA extracted from bones and teeth is often used in genetic studies to determine the presence of certain genetic mutations or markers.
Feature and Benefits
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High-quality DNA extraction: Bone and teeth are tough tissues that are difficult to liquefy, but a Bone and Teeth DNA Purification Kit provides reagents and protocols specifically designed for extracting high-quality DNA.
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Ease of use: Bone and Teeth DNA Purification Kits are easy to use and provide a straightforward process for purifying DNA from bones and teeth.
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High yield: Bone and Teeth DNA Purification Kits are designed to yield high amounts of DNA, which is important for downstream applications.
General Reference
1.
Loreille OM, Diegoli TM, Irwin JA, Coble MD, Parsons TJ. High efficiency DNA extraction from bone by total demineralization. Forensic Sci Int Genet. 2007 Jun;1(2):191-5
2.
Mckinnon M, Henneberg M, Higgins D. A review of the current understanding of burned bone as a source of DNA for human identification. Sci Justice. 2021 Jul;61(4):332-338.
3.
Jakubowska J, Maciejewska A, Pawłowski R. Comparison of three methods of DNA extraction from human bones with different degrees of degradation. Int J Legal Med. 2012 Jan;126(1):173-8.
4.
Emery MV, Bolhofner K, Winingear S, Oldt R, Montes M, Kanthaswamy S, Buikstra JE, Fulginiti LC, Stone AC. Reconstructing full and partial STR profiles from severely burned human remains using comparative ancient and forensic DNA extraction techniques. Forensic Sci Int Genet. 2020 May; 46:102272.
5.
Prediction of autosomal STR typing success in ancient and Second World War bone samples. Zupanič Pajnič I, Zupanc T, Balažic J, Geršak ŽM, Stojković O, Skadrić I, Črešnar M. Forensic Sci Int Genet. 2017 Mar; 27:17-26.