Entamoeba dispar is a species of amoeba that infects the human gastrointestinal tract. Although it is not considered as pathogenic as its closely related cousin, Entamoeba histolytica, E. dispar can still cause significant health problems in individuals with weakened immune systems. Like other parasites, E. dispar relies on a complex interplay of proteins to establish infection, evade the host immune system, and cause disease. In this article, we will explore some of the key proteins of E. dispar, including SAW760, Proliferating Cell Nuclear Antigen (PCNA), Serine-rich 25 kDa antigen protein, Herring worm-protein, Ring-infected erythrocyte surface antigen (RESA), 170 kDa surface lectin, Phosphatidylcholine transfer protein, Minor histocompatibility antigen H13, Enolase, Serine-threonine protein kinase, and Major antigen. By understanding the roles of these proteins, we can gain a better understanding of the biology of E. dispar and the potential targets for drug and vaccine development.

SAW760: A Key Player in Entamoeba Dispar Virulence
Proliferating Cell Nuclear Antigen (PCNA): A Conserved Protein Involved in DNA Replication and Repair
Serine-rich 25 kDa Antigen Protein: An Immunodominant Protein in Entamoeba Infections
Herring Worm Protein: A Potential Drug Target Against Entamoeba Infections
Ring-infected Erythrocyte Surface Antigen (RESA): A Protein Involved in Host Immune Evasion
170 kDa Surface Lectin: A Key Adhesin in Entamoeba Dispar Attachment and Invasion
Phosphatidylcholine Transfer Protein: A Protein Involved in Host Cell Membrane Modulation
Minor Histocompatibility Antigen H13: A Protein Involved in Host Immune Response Against Entamoeba Infections
Enolase: A Glycolytic Enzyme with Moonlighting Functions in Entamoeba Dispar Pathogenesis
Serine-threonine Protein Kinase: A Regulator of Entamoeba Dispar Virulence
Major Antigen: A Surface Protein Involved in Entamoeba Dispar Adherence and Invasion
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The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Entamoeba dispar cDNA and recombinant antigens can be used in a variety of applications, such as serodiagnostic tests, vaccine development, and therapeutic approaches.

In serodiagnostic tests, Entamoeba dispar cDNA and recombinant antigens can be used in ELISA tests to detect the presence of E. dispar antibodies in a sample. This can be used to diagnose E. dispar infections.

In vaccine development, Entamoeba dispar cDNA and recombinant antigens can be used to create a vaccine that helps the body create an immune response against the pathogen. This could provide protection against E. dispar infection in the future.

In therapeutic approaches, Entamoeba dispar cDNA and recombinant antigens can be used to create drugs that target the pathogen. This can help reduce the severity of the infection and the duration of symptoms.