Theileria China is a protozoan parasite that infects domestic and wild ruminants, causing a disease known as theileriosis. This disease is transmitted by ticks and affects the red blood cells of the host, leading to anemia and other serious complications. The pathogenesis of Theileria China is complex, involving various proteins and molecules that facilitate the invasion, survival, and proliferation of the parasite within the host. One of the key players in Theileria China pathogenesis is the surface protein, which plays a critical role in the interaction between the parasite and the host cell.

Surface Protein in Theileria China Pathogenesis:

Surface proteins are proteins that are localized on the outer surface of the cell and play a crucial role in cell-cell interactions, signaling, and immune evasion. In Theileria China, surface proteins have been shown to be involved in the attachment, invasion, and survival of the parasite within the host cell. Two major surface proteins that have been identified in Theileria China are TaSP and TaSd.

TaSP (Theileria annulata surface protein) is a highly polymorphic protein that is expressed on the surface of the parasite and is involved in the attachment and invasion of the host cell. It has been shown to interact with various host cell molecules, such as integrins and heparin sulfate proteoglycans, and may also play a role in modulating the host immune response. TaSP has been identified as a potential vaccine candidate for the control of Theileria China.

TaSd (Theileria annulata surface protein d) is another surface protein that is involved in the invasion of the host cell by Theileria China. It has been shown to be expressed on the surface of the parasite and to interact with host cell proteins such as CD44 and hyaluronan. TaSd may also play a role in modulating the host immune response, similar to TaSP.

Theileria China cDNA and recombinant antigen can be applied in the development of diagnostic tools and therapeutic treatments for Theileria China, a species of tick-borne protozoan parasite. The cDNA can be used to identify and characterize the gene expression of Theileria China and to identify new targets for drug development. The recombinant antigen can be used to develop an antibody-based diagnostic test for Theileria China infection. The antigen can also be used to develop a vaccine to prevent infection. Additionally, the antigen can be used to identify immune-protective antigens that can be used to develop therapeutic treatments against Theileria China.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.