Taenia solium is a Tapeworm that infects humans through the ingestion of undercooked pork contaminated with its larvae. It can cause taeniasis and cysticercosis, leading to various symptoms, including seizures, headaches, and visual impairments. Researchers have identified various antigens in Taenia solium that have diagnostic and therapeutic applications, including H17g protein, tegumental antigen, TSES38, TSES33, diagnostic antigen GP50, and antigen B (AgB).

H17g Protein: H17g protein is a tegumental antigen found in Taenia solium that plays a significant role in its infection. It has been found to induce a strong immune response in infected individuals, making it an attractive target for diagnostics and therapeutics. Studies have shown that H17g protein has a high sensitivity and specificity in detecting active cysticercosis, making it a promising diagnostic tool.

H17g protein is located in the outer layer of Taenia solium’s tegument, where it plays a critical role in the parasite’s survival and evasion of the host’s immune system. It is involved in nutrient uptake, immune evasion, and parasite-host interaction, making it a potential target for developing vaccines and therapeutics.

H17g protein has shown promising results in developing diagnostic tests for cysticercosis, including ELISA and Western blot assays. Additionally, it has potential therapeutic applications, including as a vaccine candidate, due to its ability to induce a strong immune response.

TSES38 and TSES33 Diagnostic Antigen
TSES38 and TSES33 are two diagnostic antigens found in Taenia solium that have shown high sensitivity and specificity in detecting active cysticercosis. They are glycoproteins that have been identified as potential biomarkers for developing diagnostic tests for cysticercosis.

Characteristics and Properties
TSES38 and TSES33 are found in the Taenia solium’s scolex, where they play a critical role in the parasite’s attachment and penetration into the host’s intestinal mucosa. They have been found to induce a strong immune response in infected individuals, making them potential targets for developing diagnostic tests and therapeutics.

Taenia solium cDNA and recombinant antigens have been used in various applications, such as diagnostics, vaccines, and therapeutics.

Diagnostics: Taenia solium cDNA and recombinant antigens have been used in various diagnostic tests, including ELISA, dot blotting, and western blotting, to detect and quantify Taenia solium infection. These tests have been used to detect cysticercosis, an infection caused by Taenia solium, in humans and other animals.

Vaccines: Taenia solium cDNA and recombinant antigens have been used to develop vaccines against Taenia solium infection. These vaccines have been shown to be effective in preventing cysticercosis in both humans and animals.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.