Echinococcus multilocularis is a tapeworm that can cause alveolar echinococcosis (AE), a potentially fatal disease in humans. AE occurs when the larval stage of the parasite grows and proliferates within the human liver, causing a range of symptoms including abdominal pain, jaundice, and liver failure.

Several key antigens of E. multilocularis have been identified as potential targets for the development of diagnostic tests and vaccines against AE. These include Antigen B, 6 and EM13, Em18 antigen, oncosphere antigen, and tegument protein.

Antigen B is one of the most studied antigens of E. multilocularis and is involved in the immune evasion and pathogenesis of the parasite. It has been shown to elicit a strong antibody response in infected humans, making it a potential diagnostic marker for AE.

The Em18 antigen is another important antigen of E. multilocularis, which is expressed on the surface of the parasite and is believed to play a role in host-parasite interactions. It has been shown to be immunogenic in both animal models and humans, making it a potential target for vaccine development against AE.

Other antigens of E. multilocularis, such as the oncosphere antigen and tegument protein, are also being investigated for their potential use in the development of diagnostic tests and vaccines against AE.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Echinococcus multilocularis cDNA and recombinant antigens can be used as diagnostic tools for the diagnosis of Echinococcus multilocularis infections. The cDNA from this parasite can be used to amplify specific genes and detect their presence in a sample. Recombinant antigens can be used as a rapid and specific test to detect the presence of E. multilocularis in a sample. This antigen can also be used to differentiate between different species of Echinococcus. Both cDNA and recombinant antigens can be used to diagnose the presence of E. multilocularis in humans and other animals. They can also be used in epidemiological studies to monitor the spread of this parasite and its prevalence in different locations.