Treponema denticola cDNA and recombinant antigen

Treponema denticola is a spirochete bacterium that is commonly associated with periodontal disease, a chronic inflammatory disease that affects the supporting tissues of teeth. This bacterium is a gram-negative, anaerobic, and highly motile microorganism that is found in the subgingival plaque, a biofilm that forms on the surface of teeth and gums.

One of the main virulence factors of Treponema denticola is the 53 kDa membrane antigen A (Protein A), which is a surface-exposed lipoprotein that is involved in the bacterium’s ability to colonize and invade host tissues. Protein A is a major antigen that elicits an immune response in the host and is also involved in the activation of the host’s immune system.

In addition to Protein A, Treponema denticola also produces the Cpn60 groEL protein, which is a chaperone protein that plays a role in the bacterium’s stress response. Cpn60 is a member of the chaperonin family of proteins that assists in the proper folding of other proteins in the cell. This protein is also involved in the stabilization of other virulence factors in the bacterium.

Studies have shown that the expression of Protein A and Cpn60 in Treponema denticola is regulated by environmental factors, such as pH, temperature, and oxygen tension. These proteins are essential for the survival of the bacterium in the oral cavity and its ability to cause disease.

Overall, Treponema denticola is a pathogenic bacterium that produces several proteins, including Protein A and Cpn60, that contribute to its ability to colonize and invade host tissues and evade the host’s immune response.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Treponema denticola cDNA and recombinant antigen array can be used to identify potential biomarkers of bacterial diseases. This type of array can be used in clinical, diagnostic, and research settings to detect and monitor the presence of specific bacterial antigens or genetic material in a sample. For example, it can be used to identify the presence of a specific pathogen in a sample, or to detect the presence of genetic material associated with a particular disease. Additionally, the array can be used to identify potential biomarkers of bacterial diseases, such as the presence of specific genes or proteins associated with a particular disease. This type of array has the potential to be used to diagnose and monitor bacterial infections, as well as to identify potential therapeutic targets for the treatment of bacterial diseases.