Staphylococcus saprophyticus cDNA and Antigen

Staphylococcus saprophyticus is a bacterium commonly found in the urinary tract of sexually active young women. It is a known cause of urinary tract infections and is considered an opportunistic pathogen. To cause infections, S. saprophyticus has developed a variety of virulence factors, including immunodominant antigen, staphylococcal antigen A, and secretory antigen.

Immunodominant antigen and staphylococcal antigen A are surface proteins of S. saprophyticus that are known to elicit an immune response in humans. They are considered potential vaccine candidates due to their ability to induce protective antibodies in animal models. Research has also shown that these antigens are expressed in most S. saprophyticus strains, suggesting that they may play conserved roles in the biology of this bacterium.

Secretory antigen is involved in the secretion of proteins into the extracellular environment. It is considered an important virulence factor, as it is involved in the formation of biofilms and the colonization of host tissues. Research has also shown that this antigen is involved in the regulation of genes that are important for the survival of S. saprophyticus under stress conditions.

Overall, immunodominant antigen, staphylococcal antigen A, and secretory antigen are important virulence factors of S. saprophyticus. Understanding the function of these proteins may lead to the development of novel therapeutics or vaccines for the prevention and treatment of S. saprophyticus infections.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Staphylococcus saprophyticus cDNA and recombinant antigen can be used in diagnostic tests for the detection of this pathogen in clinical samples. Detection of S. saprophyticus cDNA and recombinant antigens can be performed using nucleic acid amplification techniques such as PCR or real-time PCR. The PCR products can be detected using either a labeled probe or a fluorescent dye. These tests are highly specific and sensitive and can be used to detect the presence of the pathogen in clinical samples. In addition, immunological assays, such as ELISA and Western blot, can be used to detect S. saprophyticus-specific antigens in clinical samples. These assays are also highly specific and sensitive and can be used to detect the presence of the pathogen in clinical samples.