Shigella flexneri cDNA and Antigen

Shigella flexneri is a type of bacteria that can cause infection in humans. This bacteria has several proteins known as virulence factors that are important for its ability to cause disease. Some of the important virulence factors of Shigella flexneri include the icsA autotransporter, E3 ubiquitin-protein ligase ipaH4.5, virulence protein icsB, and invasion plasmid antigens 5a and icsA.

The icsA autotransporter is a protein that helps the bacteria move within the host cells and spread the infection. E3 ubiquitin-protein ligase ipaH4.5 is a protein that helps the bacteria evade the host immune system by interfering with the host cell’s ability to defend against the infection. Virulence protein icsB helps the bacteria survive and replicate within the host cells. Invasion plasmid antigens 5a and icsA are also important for the bacteria’s ability to invade and cause disease in the host.

These virulence factors are critical for the invasion and pathogenesis of Shigella flexneri, and are therefore important targets for research and drug development. Understanding these proteins and how they work can help in the development of effective treatments and preventive measures against infections caused by this bacterium.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Shigella flexneri cDNA and recombinant antigen can be used in the development of a diagnostic test for the detection of Shigella infections. The cDNA and antigen can be used to design primers and probes for PCR-based diagnostic tests, such as real-time PCR. The cDNA and antigen can also be used to develop enzyme-linked immunosorbent assays (ELISA) for the detection of Shigella proteins. Additionally, recombinant antigens can be used to develop rapid diagnostic tests, such as lateral flow immunoassays, to quickly and accurately detect Shigella infections.